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HTMP2MAG-54K

Millipore

MILLIPLEX® Human TIMP Magnetic Bead Panel 2 - Immunology Multiplex Assay

TIMP Bead-Based Multiplex Assays using the Luminex technology enables the simultaneous analysis of multiple TIMP biomarkers in human cell/tissue culture supernatant samples.

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About This Item

UNSPSC Code:
12161503
eCl@ss:
32161000
NACRES:
NA.84

Quality Level

200

species reactivity

human

manufacturer/tradename

Milliplex®

assay range

accuracy: 77-95%
standard curve range: 10-10,000 pg/mL
(TIMP-4)
standard curve range: 20-20,000 pg/mL
(TIMP-1)
standard curve range: 49-50,000 pg/mL
(TIMP-2)
standard curve range: 98-100,000 pg/mL
(TIMP-3)

technique(s)

multiplexing: suitable

detection method

fluorometric (Luminex xMAP)

shipped in

wet ice

General description

Tissue Inhibitors of Metalloproteinases (TIMPs) comprise a family of four inhibitors known as TIMP-1, TIMP-2, TIMP-3, and TIMP-4. Each member of the TIMP family has its own distinct profile of metalloproteinase inhibition. The matrix metalloproteinases (MMPs) are a family of zinc proteases involved in the breakdown of extracellular matrix (ECM) in normal physiological processes, such as embryonic development, tissue and bone remodeling, wound healing, tissue morphogenesis, angiogenesis, and tumor metastasis. MMPs are also known to be involved in the cleavage of cell surface receptors, the release of apoptotic ligands (such as the FAS ligand), cell proliferation, differentiation, and chemokine in/activation. The normal function of MMPs must be precisely regulated by their endogenous protein inhibitors, TIMPs . TIMPs inhibit MMPs by forming 1:1, non-covalent complexes with MMPs, thereby blocking access of substrates to the MMP catalytic site. Except for inhibition of active MMPs, TIMPs also exhibit several other biochemical and physiological/biological functions such as proMMP activation, cell growth promotion, matrix binding, inhibition of angiogenesis and the induction of apoptosis. Disruption of the MMP/TIMP balance can result in serious diseases such as arthritis, cardiovascular disorders, tumor growth and metastasis.

The MILLIPLEX® Human TIMP Panel 2 Bead kit (TIMP-1, -2, -3, -4) has been designed for tissue/cell culture samples.

The Luminex® xMAP® platform uses a magnetic bead immunoassay format for ideal speed and sensitivity to quantitate multiple analytes simultaneously, dramatically improving productivity while conserving valuable sample volume.

Panel Type: Cytokines/Chemokines

Specificity

Cross Reactivty
No significant cross-reactivity was observed among analytes in this panel.

Application

  • Analytes: TIMP-1, TIMP-2, TIMP-3, TIMP-4
  • Recommended Sample type: Tissue and cell culture supernatants
  • Assay Run Time: One day or overnight
  • Research Category: Inflammation & Immunology

Features and Benefits

Design your multiplex kit by choosing available analytes within this panel.

Storage and Stability

Recommended storage for kit components is 2 - 8°C.

Other Notes

Sensitivity: Refer to kit protocol for sensitivities of individual analytes.

Legal Information

Luminex is a registered trademark of Luminex Corp
MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany
xMAP is a registered trademark of Luminex Corp

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Pictograms

Exclamation markEnvironment
GHS07,GHS09

Signal Word

Warning

Hazard Classifications

Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Irrit. 2 - Skin Irrit. 2 - Skin Sens. 1 - STOT SE 3

Target Organs

Respiratory system

Storage Class Code

10 - Combustible liquids

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

HTMP2MAG-54K-03:
HTMP2MAG-54K:
HTMP2MAG-54K-02:
HTMP2MAG-54K-01:
HTMP2MAG-54K-04:

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Larissa Berloffa Belardin et al.
Scientific reports, 9(1), 903-903 (2019-01-31)
Matrix Metalloproteinases (MMPs) and their regulators - Tissue Inhibitors of Matrix Metalloproteinases (TIMPs) - participate in extracellular matrix remodeling, fibrosis, and semen liquefaction, as well as to inflammatory activity. Seminal plasma has been shown to contain MMPs (MMP-2 and MMP-9)
Abstracts from the 43rd American Society of Andrology Annual Meeting, 21-24 April 2018, Portland, Oregon.
Andrology, 6 Suppl 1, 1-98 (2018-03-28)
Jonathan H Soslow et al.
Journal of cardiac failure, 25(4), 259-267 (2019-02-15)
Cardiomyopathy is the leading cause of death in Duchenne muscular dystrophy (DMD). Standard cardiac biomarkers are poor indicators of DMD cardiovascular disease. Matrix metalloproteinases (MMPs) and tissue inhibitors of metalloproteinases (TIMPs) regulate collagen turnover. Given the cardiac fibrosis seen in
Julia E Herrmann et al.
Toxicology and applied pharmacology, 274(2), 302-312 (2013-11-30)
Human response to isoproterenol induced cardiac injury was evaluated by gene and protein pathway changes in human heart slices, and compared to rat heart slices and rat heart in vivo. Isoproterenol (10 and 100μM) altered human and rat heart slice
Preethi Vijayaraj et al.
Cell reports, 29(11), 3488-3505 (2019-12-12)
Progressive organ fibrosis accounts for one-third of all deaths worldwide, yet preclinical models that mimic the complex, progressive nature of the disease are lacking, and hence, there are no curative therapies. Progressive fibrosis across organs shares common cellular and molecular

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