GF037

Thrombopoietin

TPO (Thrombopoietin, Mpl-ligand, MGDF) stimulates the proliferation & maturation of megakaryocytes & promotes increased circulating levels of platelets in vivo.

• Synonym(s): TPO
• UNSPSC Code: 12352202
• eCl@ss: 32160405
• NACRES: NA.75

Properties

• biological source: human
• Quality Level: 100
• Assay: >98% (SDS-PAGE and HPLC)
• specific activity: ≥1x10(e6) U/mg
• manufacturer/tradename: Chemicon^®
• technique(s): cell culture | mammalian: suitable
• impurities: <0.1 ng/mg Endotoxin (of human TPO; 1 EU/μg)
• input: sample type hematopoietic stem cell(s)
• NCBI accession no.: NM_000460.2; NM_199228.1; NM_199356.1
• UniProt accession no.: P40225
• shipped in: dry ice
• Gene Information: human ... TPO(7173)

Description

General description

Product Source: E.coli

TPO (Thrombopoietin, Mpl-ligand, MGDF) stimulates the proliferation and maturation of megakaryocytes and promotes increased circulating levels of platelets in vivo. Chemicon′s Human TPO is a soluble 18.5 kDa protein containing 174 amino acid residues which comprises the receptor binding domain of the Mpl-ligand protein.

Biochem/physiol Actions

The ED50 was determined by the dose-dependent stimulation of the proliferation of M07e cells is ≤1.0 ng/mL

Physical form

Lyophilized protein from 5mM Tris, pH 7.6, with no additives.

Storage and Stability

The lyophilized powder, though stable at room temperature, is best stored at -20°C. Reconstitute in 5 mM Tris, pH 8.0 to a concentration of 0.5-1.0 mg/mL. This solution can be diluted into other aqueous buffers and stored at 4ºC for 1 week or -20ºC for future use. Reconstituted TPO should be stored in working aliquots at -20°C.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1