Skip to Content
Merck
All Photos(1)

Documents

Safety Information

ABE86

Sigma-Aldrich

Anti-CHD-3 Antibody

from rabbit, purified by affinity chromatography

Synonym(s):

Chromodomain-helicase-DNA-binding protein 3, CHD-3, ATP-dependent helicase CHD3, Mi-2 autoantigen 240 kDa protein, Mi2-alpha, Zinc finger helicase, hZFH

Sign Into View Organizational & Contract Pricing
All Photos(1)

About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

100

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

human

technique(s)

western blot: suitable

NCBI accession no.

NP_001005273

UniProt accession no.

Q12873

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... CHD3(1107)

General description

Chromodomain-helicase-DNA-binding protein 3, more commonly known as CHD-3, is a member of the CHD family of proteins. These proteins are characterized by the presence of chromo (chromatin organization modifier) domain, SNF2-related helicase/ATPase domain, and and a carboxy-terminal DNA binding domain. As a component of the Mi-2/NuRD histone deacetylase complex, CHD-3 participates in chromatin remodeling by deacetylating histones.

Specificity

This antibody recognizes CHD-3 at the C-terminus.

Immunogen

Epitope: C-terminus
KLH-conjugated linear peptide corresponding to the C-terminus of human CHD-3.

Application

Anti-CHD-3 Antibody is a Rabbit Polyclonal Antibody for detection of CHD-3 also known as Chromodomain-helicase-DNA-binding protein 3, hZFH & has been validated in WB.
Research Category
Epigenetics & Nuclear Function
Research Sub Category
Chromatin Biology

Quality

Evaluated by Western Blot in HeLa cell lysate.

Western Blot Analysis: 0.05 µg/mL of this antibody detected CHD-3 on 10 µg of HeLa cell lysate.

Target description

~ 250 kDa observed

Linkage

Replaces: MABE482

Physical form

Affinity purified
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
HeLa cell lysate

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Not finding the right product?  

Try our Product Selector Tool.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

ABE86:

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Zhiliang Fan et al.
Molecular medicine reports, 18(1), 169-178 (2018-05-12)
Epilepsy is a type of refractory neurologic disorder mental disease, which is associated with cognitive impairments and memory dysfunction. However, the potential mechanisms of epilepsy are not well understood. Previous evidence has identified the voltage gated potassium channel complex (VGKC)
Xiaoqin Hu et al.
Experimental and therapeutic medicine, 15(2), 1795-1802 (2018-02-13)
Alzheimer's disease is associated with cognitive impairments that affect memory and executive functions. Simvastatin is a cholesterol-lowering statin drug that is used to control levels of cholesterol in the blood, particularly in cases of hypercholesterolemia, and may be used in
Lauren M Wagner et al.
PloS one, 8(10), e78242-e78242 (2013-10-23)
The herpes simplex virus type 1 (HSV-1) immediate early protein, ICP4, participates in the regulation of viral gene expression by both activating and repressing RNA polII transcription. We used affinity purification of ICP4 expressed in infected cells followed by mass

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service