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ABE1845

Sigma-Aldrich

Anti-GABPA Antibody

from rabbit

Synonym(s):

GA-binding protein alpha chain, GABP subunit alpha, GABPA

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

100

antibody form

purified antibody

antibody product type

primary antibodies

clone

polyclonal

species reactivity

human, rat, mouse

species reactivity (predicted by homology)

monkey (based on 100% sequence homology), bovine (based on 100% sequence homology), canine (based on 100% sequence homology)

technique(s)

ChIP: suitable
western blot: suitable

NCBI accession no.

NP_032091

UniProt accession no.

Q00422

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... GABPA(2551)
mouse ... Gabpa(14390)

General description

GA-binding protein alpha chain (UniProt Q00422; also known as GA repeat binding protein alpha, GABP subunit alpha, GABPalpha, NRF-2, Nuclear respiratory factor-2) is encoded by the Gabpa gene (also known as E4tf1a) in murine species (Gene ID 14390). GABPalpha is a redox-responsive transcription factor that plays an anti-oxidant, pro-survival role in mitochondrial biogenesis process. GABPalpha is upregulated in response to oxidant exposure or increased cellular reactive oxygen species (ROS), where it mediates the transcription of mitochondrial transcription factor A (mtTFA) that is needed for mitochondrial DNA transcription.

Immunogen

Epitope: Near C-terminus
KLH-conjugated linear peptide corresponding to mouse GABPA near the C-terminus.

Application

Detect GABPA using this Anti-GABPA Antibody validated for use in Western Blotting and Chromatin Immunoprecipitation (ChIP).
Research Category
Epigenetics & Nuclear Function
Research Sub Category
Nuclear Receptors
Western Blotting Analysis: A representative lot detected GABPA in mouse liver nuclear proteins (Cherry, A.D., et al. (2014). J. Biol. Chem. 289(1):41-52).
Chromatin Immunoprecipitation Analysis: A representative lot detected GABPA occupancy at the Sod2 promoter region by ChIP using liver chromatin preparation from mice after S. aureus inoculation (Cherry, A.D., et al. (2014). J. Biol. Chem. 289(1):41-52).

Quality

Evaluated by Western Blotting in mouse liver tissue lysate.

Western Blotting Analysis: 2.0 µg/mL of this antibody detected GABPA in 10 µg of mouse liver tissue lysate.

Target description

~55 kDa observed

Physical form

Format: Purified
Protein A purified
Purified rabbit polyclonal antibody in buffer containing PBS and 0.01% Sodium Azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

ABE1845:

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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