ABE1325

Anti-FOXK1 (MNF)

from rabbit, purified by affinity chromatography

• Synonym(s): Forkhead box protein K1, Myocyte nuclear factor, MNF
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: rabbit
• Quality Level: 100
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• purified by: affinity chromatography
• species reactivity: human
• packaging: antibody small pack of 25 μg
• technique(s): immunohistochemistry: suitable (paraffin); western blot: suitable
• isotype: IgG
• NCBI accession no.: NP_001032242.1
• UniProt accession no.: P85037
• shipped in: ambient
• target post-translational modification: unmodified
• Gene Information: human ... FOXK1(221937)

Description

General description

Forkhead box protein K1 (UniProt: P85037; also known as Myocyte nuclear factor, MNF) is encoded by the FOXK1 (also known as MNF) gene (Gene ID: 221937) in human. Forkhead box protein K1 is a transcriptional regulator that binds to the upstream enhancer region (CCAC box) of myoglobin gene. It is an important regulatory factor of the myogenic progenitor cell population. It is involved in the cell cycle process and promotes proliferation by repressing Foxo4 transcriptional activity and the cyclin-dependent kinase inhibitor, p21CIP, in the myogenic progenitor cells. It is shown to represses myogenic differentiation by inhibiting MEFC activity and has a role in remodeling processes of adult muscles that occur in response to physiological stimuli. It is shown to be essential for correct temporal orchestration of molecular and cellular events necessary for muscle repair. Forkhead box protein K1 is expressed both developing and adult tissues. In adults, significant expression is seen in tumors of the brain, colon, and lymph node. Two isoforms of Forkhead box protein K1 have been reported that are produced by alternative splicing. Both FOXK1 and FOXK2 proteins are shown to act as transcriptional repressors of autophagy in muscle cells and fibroblasts. They specifically recruit Sin3A HDAC complexes to restrict acetylation of histone H4 and expression of critical autophagy genes. The loss of FOXK1 and FOXK2 proteins results in the upregulation of several genes controlling the initiation and nucleation of autophagosome formation. (Ref.: Bowman, CJ., et al. (2014). Nature Cell Biology 16(12); 1202-1214).

Specificity

This rabbit polyclonal antibody detects Forkhead box protein K1 in human cells. It targets an epitope within 20 amino acids from the C-terminal region.

Immunogen

Epitope: C-terminus

KLH-conjugated linear peptide corresponding to 20 amino acids from the C-terminal region of human Forkhead box protein K1.

Application

Anti-FOXK1 (MNF), Cat. No. ABE1325, is a highly specific rabbit polyclonal antibody that targets Forkhead box protein K1 and has been tested in Immunohistochemistry (Paraffin) and Western Blotting.

Research Category
Epigenetics & Nuclear Function

Western Blotting Analysis: 2 µg/mL from a representative lot detected FOXK1 (MNF) in HepG2 cell lysate.
Immunohistochemistry Analysis: A 1:250 dilution from a representative lot detected FOXK1 (MNF) in human cerebral cortex, human liver, and human testis tissues.

Western Blotting Analysis: 2 µg/mL from a representative lot detected FOXK1 (MNF) in HepG2 cell lysate.

Immunohistochemistry Analysis: A 1:250 dilution from a representative lot detected FOXK1 (MNF) in human cerebral cortex, human liver, and human testis tissues.

Quality

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: 2 µg/mL of this antibody detected FOXK1 (MNF) in HeLa cell lysate.

Target description

~95 kDa observed; 75.46 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Physical form

Affinity Purified

Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point(F): does not flash
• Flash Point(C): does not flash