AB5744

Anti-Nkx-5.1 Antibody

Chemicon^®, from rabbit

• Synonym(s): NK-2
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: rabbit
• Quality Level: 100
• antibody form: saturated ammonium sulfate (SAS) precipitated
• antibody product type: primary antibodies
• clone: polyclonal
• purified by: affinity chromatography
• species reactivity: mouse, human
• manufacturer/tradename: Chemicon^®
• technique(s): immunohistochemistry: suitable; western blot: suitable
• NCBI accession no.: NM_001105574.1
• UniProt accession no.: A6NHT5
• shipped in: dry ice
• target post-translational modification: unmodified
• Gene Information: mouse ... Hmx3(15373)

Description

General description

The previously assigned protein identifier A8MU06 has been merged into A6NHT5. Full details can be found on the UniProt database.

Specificity

Recognizes Nkx5.1, (NK-2 family), a homeodomain transcription factor.

Immunogen

Synthetic peptide, amino acids 10-23 from mouse Nkx5.1.

Application

Research Category
Neuroscience

Research Sub Category
Developmental Neuroscience

Neuronal & Glial Markers

This Anti-Nkx-5.1 Antibody is validated for use in WB, IH for the detection of Nkx-5.1.

Western blot: 1:200-1:1,000 using ECL.

Immunohistochemistry: 1:200-1:1,000.

Optimal working dilutions must be determined by the end user.

Physical form

Precipitated antibody in a solution of 50% saturated ammonium sulfate and PBS containing no preservatives.

Storage and Stability

Maintain unopened vial at -70°C for up to 6 months. Avoid repeated freeze/thaw cycles.

PREPARATION AND USE:

To reconstitute the antibody, centrifuge the antibody vial at moderate speed (5,000 rpm) for 5 minutes to pellet the precipitated antibody product. Carefully remove the ammonium sulfate/PBS buffer solution and discard. It is not necessary to remove all of the ammonium sulfate/PBS solution: 10 μL of residual ammonium sulfate solution will not affect the resuspension of the antibody. Do not let the protein pellet dry, as severe loss of antibody reactivity can occur.

Resuspend the antibody pellet in a suitable biological buffer such as PBS or TBS (pH 7.3-7.5) to a final concentration of 1.0 mg/mL. For example, to achieve a 1.0 mg/mL concentration with 50 μg of precipitated antibody, the amount of buffer needed would be 50 μL.

Carefully add the liquid buffer to the pellet. DO NOT VORTEX. Mix by gentle stirring with a wide pipet tip or gentle finger-tapping. Let the precipitated antibody rehydrate for 1 hour at 4-25°C prior to use. Small particles of precipitated antibody that fail to resuspend are normal. Vials are overfilled to compensate for any losses.

The rehydrated antibody solutions can be stored undiluted at 2-8°C for 2 months without any significant loss of activity. Note, the solution is not sterile, thus care should be taken if product is stored at 2-8°C.

For storage at -20°C, the addition of an equal volume of glycerol can be used, however, it is recommended that ACS grade or higher glycerol be used, as significant loss of activity can occur if the glycerol used is not of high quality.

For long-term storage at -70°C, it is recommended that the rehydrated antibody solution be further diluted 1:1 with a 2% BSA (fraction V, highest-grade available) solution made with the rehydration buffer. The resulting 1% BSA/antibody solution can be aliquoted and stored frozen at -70°C for up to 6 months. Avoid repeated freeze/thaw cycles.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable