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AB5532

Sigma-Aldrich

Anti-Nitrotyrosine Antibody

serum, Chemicon®

Synonym(s):

NO2-Tyrosine

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

antibody form

serum

antibody product type

primary antibodies

clone

polyclonal

species reactivity (predicted by homology)

all

manufacturer/tradename

Chemicon®

technique(s)

ELISA: suitable
immunohistochemistry: suitable

shipped in

dry ice

Gene Information

human ... NOS1(4842)

General description

The cellular production of highly reactive nitrogen species derived from nitric oxide, such as peroxynitrite, nitrogen dioxide and nitryl chloride, leads to the nitration of tyrosine resides in tissue proteins. The extent of protein nitrotyrosine formation provides an index of the production of reactive nitrogen species and potential cell damage over a period of time. Nitrotyrosine can be measured by amino-acid analysis of protein hydrolysates and detected, estimated semi-quantitatively and located in cells and tissues by immunocytochemical techniques using antibodies directed against the nitrotyrosine hapten.

Specificity

NO2-Tyrosine



The cross-reactivities were determined using an ELISA test by competition experiments with the following compounds:



Compound Cross-reactivity ratio

NO2-Tyrosine-G-BSA 1

NO-Tyr-G-BSA 1/233

Tyr-G-BSA 1/>100,000

NO2-5HT-G-BSA 1/>100,000

NO-5HT-G-BSA 1/>100,000

NO-Cys-G-BSA 1/>100,000

NO-W-G-BSA 1/>100,000

NO-His-G-BSA 1/>100,000



Abbreviations:



(G) Glutaraldehyde

(BSA) Bovine Serum Albumin

Immunogen

NO2-Tyrosine-Glutaraldehyde-BSA

Application

Anti-Nitrotyrosine Antibody is an antibody against Nitrotyrosine for use in ELISA, IH.
Immunohistochemistry: 1:1,000-1:2,500

Optimal working dilutions must be determined by the end user.
Research Category
Neuroscience
Research Sub Category
Oxidative Stress

Physical form

Rabbit antiserum. Liquid with 0.05% sodium azide.

Storage and Stability

Maintain at -20°C in undiluted aliquots for up to 12 months. Avoid repeated freeze/thaw cycles.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

AB5532:

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Maria A Pabón et al.
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Chronic obstructive pulmonary disease (COPD), associated with cigarette smoke-induced (CS-induced) emphysema, contributes significantly to the global health care burden of disease. Although chronic kidney disease (CKD) may occur in patients with COPD, the relationship between COPD and CKD remains unclear.
S Y Yao et al.
Journal of neuroimmunology, 229(1-2), 146-156 (2010-08-21)
There are currently four known isoforms of nitric oxide synthase (NOS). Of these, neuronal NOS (nNOS) is known to be present exclusively in neurons, endothelial NOS (eNOS) in vascular endothelium, while the inducible form of NOS (iNOS) is known to
Usua Laresgoiti et al.
Development (Cambridge, England), 143(20), 3686-3699 (2016-11-02)
Insufficient alveolar gas exchange capacity is a major contributor to lung disease. During lung development, a population of distal epithelial progenitors first produce bronchiolar-fated and subsequently alveolar-fated progeny. The mechanisms controlling this bronchiolar-to-alveolar developmental transition remain largely unknown. We developed

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