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AB2257

Sigma-Aldrich

Anti-Vesicular GABA Transporter Antibody

serum, from rabbit

Synonym(s):

GABA and glycine transporter, Solute carrier family 32 member 1, Vesicular GABA transporter, solute carrier family 32 (GABA vesicular transporter), member 1, solute carrier family 32, member 1, vesicular inhibitory amino acid transporter

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

100

antibody form

serum

antibody product type

primary antibodies

clone

polyclonal

species reactivity

rat

species reactivity (predicted by homology)

monkey, human

technique(s)

immunohistochemistry: suitable
western blot: suitable

NCBI accession no.

NM_080552.2

UniProt accession no.

Q9H598

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... SLC32A1(140679)
mouse ... Slc32A1(22348)

General description

Vesicular GABA transporter (VGAT), the first vesicular amino acid transporter to be molecularly identified, differs in structure from previously cloned vesicular neurotransmitter transporters and defines a novel gene family. VGAT is highly concentrated in the nerve endings of GABAergic neurons in the brain and spinal cord but also in glycinergic nerve endings. In contrast, hippocampal mossy fiber boutons, which are glutamatergic but known to contain GABA, lack VGAT immunoreactivity. Although the great majority of nerve terminals containing GABA or glycine are immunopositive for VGAT, subpopulations of nerve endings rich in GABA or glycine appear to lack the protein. Additional vesicular transporters or alternative modes of release may therefore contribute to the inhibitory neurotransmission mediated by these two amino acids.

Specificity

Cat. # AB2257 recognizes VGAT.

Immunogen

Epitope: C-terminus
KLH conjugated Vesicular GABA Transporter corresponding to the C-terminus.

Application

Anti-Vesicular GABA Transporter Antibody is an antibody against Vesicular GABA Transporter for use in WB, IH.
Immunohistochemistry Analysis:
1:50 - 1:500 dilution from a previous lot detected vesiluar GABA transporter using IHC-Select Detection with HRP-DAB.
Research Category
Neuroscience
Research Sub Category
Ion Channels & Transporters

Quality

Routinely evaluated by Western Blot on rat brain lysates.

Western Blot Analysis:
1:500 - 1:1,000 dilution of this lot detected vesicular GABA transporter on 10 µg of rat brain lysate.

Target description

Approx. 57 kDa

Physical form

Antigen Affinity Purified
Rabbit polyclonal serum in buffer containing 0.1M Tris-Glycine (pH7.4) 15mM NaCl with 0.05% NaN3.

Storage and Stability

Stable for 1 year at 2-8ºC from date of receipt.

Analysis Note

Control
Rat brain lysate.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

AB2257:

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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