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AB10911

Sigma-Aldrich

Anti-Osteocalcin Antibody

serum, from rabbit

Synonym(s):

Bone Gla protein, Gamma-carboxyglutamic acid-containing protein, bone gamma-carboxyglutamate (gla) protein (osteocalcin)

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

100

antibody form

serum

antibody product type

primary antibodies

clone

polyclonal

species reactivity

human, rat, mouse

technique(s)

immunohistochemistry: suitable
western blot: suitable

NCBI accession no.

NM_199173.3

UniProt accession no.

P02818

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... BGLAP(632)
mouse ... Bglap(12096)
rat ... Bglap(25295)

General description

Osteocalcin belongs to the osteocalcin/matrix Gla protein family. It constitutes 1 to 2% of the total bone protein. It is made by osteoblasts, and is a major component of the noncollagenous bone matrix. It has a high affinity for calcium and binds strongly to apatite. Sixty to ninety percent of de novo synthesized osteocalcin is incorporated into the bone matrix where it binds to hydroxyapatite during matrix mineralization. The remainder of the osteocalcin is released into the circulation where it can be measured as a sensitive marker of bone formation. Osteocalcin found in serum is almost exclusively derived from the bone formation with little or no contribution from the resorption process. Drugs acting primarily on bone resorption, such as bisphosphonates, induce a rapid decline in specific bone resorption markers, whereas osteocalcin levels only change after extended therapy periods, in accordance with the expected dynamics of a bone formation marker. Serum osteocalcin is elevated in diseases characterized by increased bone turnover such as osteoporosis, hyperparathyroidism and Paget′s disease, and low in conditions associated with low bone turnover such as hypoparathyroidism and growth hormone deficiency.

Specificity

The antibody reacts with the osteocalcin protein.

Immunogen

Epitope: aa 59-74
Synthetic peptide corresponding to aa 59-74 of osteocalcin protein.

Application

Detect Osteocalcin using this Anti-Osteocalcin Antibody validated for use in WB, IH.

Quality

Routinely evaluated by Western Blot on human heart lysate.

Western Blot Analysis:
1:500 - 1:5,000 dilution of this antibody detected osteocalcin in 10 μg of human heart lysate.

Target description

11 kDa calculated.
*Band of ~22 kDa is observed. The difference between observed and expected band may be due to post-translational modifications.

Linkage

Replaces: MABD182

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Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

AB10911:

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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