Skip to Content
Merck
All Photos(1)

Documents

Safety Information

70239-M

Millipore

His•Bind® Purification Kit

Synonym(s):

IMAC, Protein purification

Sign Into View Organizational & Contract Pricing
All Photos(1)

About This Item

UNSPSC Code:
41106500
NACRES:
NA.56

manufacturer/tradename

Novagen®

Quality Level

400

storage condition

OK to freeze

storage temp.

2-8°C

General description

His•Bind® purification kit includes pre-tested buffers specifically designed for IDA-based His•Bind Resin, enabling convenient and rapid one-step purification of proteins by metal affinity chromatography. It uses the His•Tag®/His•Bind® technology, that leverages the affinity between neighboring histidine in the His•Tag sequence and immobilized metal ions (such as Ni2+ or Co2+).

Application

His•Bind® Purification Kit has been used for: the purification of N-terminal 6-histidine-tagged aldehyde dehydrogenase isozyme, ALDH1A1 under nondenaturing conditions by nickel-NTA affinity chromatography, the purification of virus binding protein specific for Poliovirus Type 1, the purification of recombinant oE7, huhsp70 and oE7/huhsp70 proteins, to study the effective antitumor efficacy of human hsp70 and HPV16 oE7 fusion protein vaccine, the purification of recombinant exFPR1, MTBHsp70 and MTBHsp70-exFPR1 fusion proteins to study their role in cervical cancer immunotherapy

Features and Benefits

  • Includes His•Bind® Resin, His•Bind® buffer kit and chromatography columns.
  • Solutions are included for Ni2+ charging, binding, washing, and elution.
  • Columns are pre-packed with 1.25 ml bed volume of Ni2+-charged His•Bind Resin.

Components

•10 mlHis•Bind Resin

•1His•Bind Buffer Kit

•pkg/4Chromatography Columns

Warning

Toxicity: Multiple Toxicity Values, refer to MSDS (O)

Other Notes

Due to the nature of the Hazardous Materials in this shipment, additional shipping charges may be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.

Legal Information

HIS-BIND is a registered trademark of Merck KGaA, Darmstadt, Germany
NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany

Signal Word

Danger

Hazard Classifications

Aquatic Chronic 2 - Carc. 1A Inhalation - Eye Dam. 1 - Flam. Liq. 3 - Muta. 2 - Repr. 1B - Resp. Sens. 1 - Skin Corr. 1C - Skin Sens. 1 - STOT RE 1 - STOT RE 2 Inhalation

Target Organs

respiratory tract irritation

Storage Class Code

3 - Flammable liquids

Flash Point(F)

86.0 °F

Flash Point(C)

30 °C

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

FSL

Group 4: Flammable liquids
Type 2 petroleums
Hazardous rank III
Water insoluble liquid

JAN Code

70239-3:

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

MTBHsp70-exFPR1-pulsed Dendritic Cells Enhance the Immune Response against Cervical Cancer
Cao G, et al.
Journal of Cancer, 10(25), 6364?6373-6364?6373 (2019)
Human hsp70 and HPV16 oE7 fusion protein vaccine induces an effective antitumor efficacy
Jinbao Zong, et al.
Oncology Reports, 30(1), 407-412 (2013)
A bioluminescence assay for aldehyde dehydrogenase activity
Duellman SJ, et al.
Analytical Biochemistry, 434(2) (2013)
Construction of a Cloning System for the Mass Production of a Virus-Binding Protein Specific for Poliovirus Type 1
Sano D and Omura T
Applied and Environmental Microbiology, 71(5) (2005)

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service