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69450-M

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BL21(DE3) Competent Cells - Novagen

Escherichia coli, rod shaped

Synonym(s):

BL21 strain

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About This Item

UNSPSC Code:
41106202
NACRES:
NA.81

product name

BL21(DE3) Competent Cells - Novagen, BL21(DE3) is a chemically competent E. coli cell suitable for transformation and high level protein expression using a T7 RNA polymerase-IPTG induction system.

biological source

Escherichia coli

Quality Level

100

manufacturer/tradename

Novagen®

storage condition

OK to freeze
avoid repeated freeze/thaw cycles

growth mode

adherent or suspension

morphology

rod shaped

technique(s)

microbiological culture: suitable

cell transformation

transformation efficiency: >2×107 cfu/μg

shipped in

dry ice

storage temp.

−70°C

Related Categories

General description

A common method for transformation of DNA plasmids into E. coli is the use of chemically competent cells. Although competent cells can be prepared in the laboratory, greater efficiency, reproducibility, and convenience are achieved using Novagen prepared competent cells. Novagen competent cells represent the widest selection available for protein expression. Every Novagen competent cell strain is verified for phenotype and purity, and is guaranteed fortransformation efficiency.
T7 expression strains are lysogens of bacteriophageDE3, as indicated by the (DE3). These hosts carry a chromosomal copy of the T7 RNA polymerase gene under control of the lacUV5 promoter. Such strains are suitable for production of protein from target genes cloned in appropriate T7 expression vectors, using IPTG as an inducer.
BL21 has been the gold standard for protein expression since it was first introduced in 1990. Deficient in lon and ompT proteases, BL21 and its derivatives are ideal for many applications.
BL21(DE3) is a chemically competent E. coli cell suitable for transformation and high level protein expression using a T7 RNA polymerase-IPTG induction system.
This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges us to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.

Components

0.4 ml1 mlComponent

•2 × 0.2 ml5 × 0.2 mlBL21(DE3) Competent Cells

•2 × 2 ml4 × 2 mlSOC Medium

•2 ng2 ngTest Plasmid

Warning

Toxicity: Multiple Toxicity Values, refer to MSDS (O)

Legal Information

NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

ISHL Notified Names

Substances Subject to be Notified Names

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Biofilm dispersal contributes to bacterial spread and disease transmission. However, its exact mechanism, especially that in the pathogen Mycobacterium tuberculosis, is unclear. In this study, the cellulase activity of the M. tuberculosis Rv0062 protein was characterized, and its effect on
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Current biology : CB, 32(23), 5189-5199 (2022-11-18)
LIM-domain-containing repeat (LCR) proteins are recruited to strained actin filaments within stress fibers in cultured cells,1,2,3 but their roles at cell-cell junctions in living organisms have not been extensively studied. Here, we show that the Caenorhabditis elegans LCR proteins TES-1/Tes
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The COVID-19 pandemic has resulted in 198 million reported infections and more than 4 million deaths as of July 2021 (covid19.who.int). Research to identify effective therapies for COVID-19 includes: (1) designing a vaccine as future protection; (2) de novo drug
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Structure (London, England : 1993), 28(11), 1238-1248 (2020-08-20)
A host of new technologies are under development to improve the quality and reproducibility of cryoelectron microscopy (cryoEM) grid preparation. Here we have systematically investigated the preparation of three macromolecular complexes using three different vitrification devices (Vitrobot, chameleon, and a
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Molecular cell, 72(2), 263-274 (2018-09-25)
Antibiotic-resistant bacterial pathogens pose an urgent healthcare threat, prompting a demand for new medicines. We report the mode of action of the natural ansamycin antibiotic kanglemycin A (KglA). KglA binds bacterial RNA polymerase at the rifampicin-binding pocket but maintains potency

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