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676490

Sigma-Aldrich

VEGFR2D1-7, Human, Recombinant, S. frugiperda

Synonym(s):

Vascular Endothelial Growth Factor Receptor 2 D1-7, Soluble KDR/Flk-1

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About This Item

UNSPSC Code:
12352202
NACRES:
NA.77

Assay

≥90% (SDS-PAGE)

Quality Level

100

form

lyophilized

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze

solubility

aqueous buffer: ≥100 μg/mL
water: ≥100 μg/mL

shipped in

wet ice

storage temp.

−70°C

General description

Recombinant, human VEGFR-2 consisting of all 7 immunoglobulin-like extracellular domains, but lacking the single transmembrane region and the intracellular split tyrosine kinase domain expressed in S. frugiperda insect cells. Binding of ligand to the full-length VEGFR-2 results in a high signaling activity and mitogenic activity in endothelial cells.
Recombinant, human VEGFR-2 consisting of all 7 immunoglobulin-like extracellular domains, but lacking the single transmembrane region and the intracellular split tyrosine kinase domain expressed in S. frugiperda insect cells. Contains all of the domains necessary for high affinity binding to VEGF. VEGFR-2 has a lower affinity for VEGF165 than VEGFR-1. However, VEGFR-2 has a higher signaling activity and is reported to mediate almost all observed endothelial cell responses to VEGF, including the increased microvascular permeability and endothelial cell proliferation. VEGFR-2 requires heparin for binding VEGF165.

Biochem/physiol Actions

Measured by the ability to inhibit VEGF165-induced proliferation in human umbilical vein endothelial (HUVE) cells

Warning

Toxicity: Standard Handling (A)

Physical form

Lyophilized from 100 mM NaCl, 25 mM MES, pH 5.5.

Reconstitution

Following reconstitution, aliquot and freeze (-70°C). Stock solutions are stable for up to 6 months at -70°C. Avoid freeze/thaw cycles of solutions.

Other Notes

Robinson, C.J., and Stringer, S.E. 2001 J. Cell Sci.114, 853.
Roeckl, W., et al. 1998. Exp. Cell Res.241, 161.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

676490-100UG:
676490-200UG:
676490-500UG:
676490-800UG:
676490-0UG:
676490-UG:
676490-50UG:

Certificates of Analysis (COA)

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Melis Denizci Öncü et al.
Biotechnology and applied biochemistry, 69(5), 2122-2137 (2021-10-26)
Development of monoclonal antibody therapeutics against vascular endothelial growth factor receptor 2 (VEGFR-2) protein, which is the main regulator in angiogenesis, has been a major challenge for years. In the current study, we engineer an inclusion body forming single-chain variable

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