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662057

Sigma-Aldrich

Ubiquitin, GST-Fusion, Recombinant, E. coli

Synonym(s):

GST-Ub, no change

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About This Item

UNSPSC Code:
12352202
NACRES:
NA.77

Assay

≥95% (SDS-PAGE)

Quality Level

100

form

liquid

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
avoid repeated freeze/thaw cycles

technique(s)

cell based assay: suitable

NCBI accession no.

M26880

shipped in

wet ice

storage temp.

−70°C

General description

Research area: Cancer

Recombinant ubiquitin fused at the N-terminus to GST and expressed in E. coli. Useful for glutathione affinity purification of ubiquitinated molecules. Can also be used for immunodetection of conjugates using GST antibodies. Facilitates the visualization of polyUb chains due to larger ladder intervals.

Application

Ubiquitin, GST-Fusion, Recombinant, E. coli has been used for in vivo and in vitro ubiquitination assays, to study the role of FBXL2-Grp94-EGFR axis in non-small cell lung cancer (NSCLC) development and the role of F-box protein 3 (FBXO3) in promotion of breast cancer metastasis.

Biochem/physiol Actions

The ubiquitin (Ub) system plays a crucial role in protein homeostasis by maintaining protein balance in pathways related to DNA repair, cell cycle progression, apoptosis (programmed cell death), receptor-mediated endocytosis, and signal transduction. Imbalances or dysfunctions within this system can lead to pathological conditions. Apart from this, disruptions in ubiquitin pathways can also contribute to the development of human diseases, including neurodegenerative conditions and tumors.

Packaging

Please refer to vial label for lot-specific concentration.

Warning

Toxicity: Standard Handling (A)

Physical form

In 100 mM NaCl, 50 mM HEPES, pH 8.0.

Reconstitution

Following initial thaw, aliquot and freeze (-70°C).

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

662057-MG:
662057-1MG:

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Metalloregulation of Protein Clearance: New Therapeutic Avenues for Neurodegenerative Diseases
Munoz ET, et al.
Abstr. Soc. Neurosci (2017)
Ubiquitin networks in cancer
Kirkin V and Dikic I
Current Opinion in Genetics & Development, 21(1), 21-28 (2011)
Ubiquitin
Kona F.R, et al.
Brenner and Rector's The Kidney E-book, 242-245 (2013)
Mengmeng Niu et al.
PLoS biology, 19(2), e3001113-e3001113 (2021-02-25)
Transforming growth factor-β (TGF-β) signaling plays a critical role in promoting epithelial-to-mesenchymal transition (EMT), cell migration, invasion, and tumor metastasis. ΔNp63α, the major isoform of p63 protein expressed in epithelial cells, is a key transcriptional regulator of cell adhesion program
Mengmeng Niu et al.
Nature communications, 12(1), 5919-5919 (2021-10-13)
Abnormal activation of epidermal growth factor receptor (EGFR) drives non-small cell lung cancer (NSCLC) development. EGFR mutations-mediated resistance to tyrosine-kinase inhibitors (TKIs) is a major hurdle for NSCLC treatment. Here, we show that F-box protein FBXL2 targets EGFR and EGFR

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