539660

PKCε, Human, Recombinant, S. frugiperda

• Synonym(s): Protein Kinase Cε
• UNSPSC Code: 12352202
• NACRES: NA.77

Properties

• Assay: ≥85% (SDS-PAGE)
• Quality Level: 100
• form: liquid
• specific activity: ≥800 units/mg protein
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze; avoid repeated freeze/thaw cycles
• shipped in: wet ice
• storage temp.: −70°C

Description

General description

Full-length, recombinant, human, PKCε expressed in S. frugiperda insect cells using a baculovirus expression system. Suitable for studies of PKCε and its regulation. PKCε is a Ca²⁺-independent and phospholipid-dependent enzyme.

Packaging

Please refer to vial label for lot-specific concentration.

Warning

Toxicity: Standard Handling (A)

Unit Definition

One unit is defined as the amount of enzyme that will transfer 1 nmol phosphate to PKC_ε Peptide Substrate (Cat. No. 539562) per min at 30°C, pH 7.4.

Physical form

In 250 mM NaCl, 20 mM HEPES, 5 mM DTT, 2 mM EDTA, 2 mM EGTA, 50% glycerol, 0.05% TRITON^™ X-100 Detergent, pH 7.4.

Reconstitution

Following initial thaw, aliquot and freeze (-70°C).

Other Notes

Newton, A.C. 1995. J. Biol. Chem.270, 28495.
Dekker, L.V., and Parker, P.J. 1994. Trends Biochem. Sci. 19, 73.
Nishizuka, Y. 1992. Science 258, 607.
Bell, R.M., and Burns, D.J. 1991. J. Biol. Chem.266, 4661.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable