5.04859

2-N-Hexadecanoylamino-4-nitrophenylphosphorylcholine

≥98% (TLC), solid, acid sphingomyelinase substrate, Calbiochem^®

• Synonym(s): 2-N-Hexadecanoylamino-4-nitrophenylphosphorylcholine, HDA-PC
• CAS Number: 60438-73-5
• MDL number: MFCD00051274
• UNSPSC Code: 12352200
• NACRES: NA.77

Properties

• Product Name: 2-N-Hexadecanoylamino-4-nitrophenylphosphorylcholine,
• Assay: ≥98% (TLC)
• Quality Level: 100
• form: solid
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze; protect from light
• color: off-white
• solubility: methanol: 25 mg/mL
• storage temp.: −20°C
• SMILES string: [P](=O)([O-])(OCC[N+](C)(C)C)Oc1c(cc(cc1)[N+](=O)[O-])NC(=O)CCCCCCCCCCCCCCC.O
• InChI: 1S/C27H48N3O7P.H2O/c1-5-6-7-8-9-10-11-12-13-14-15-16-17-18-27(31)28-25-23-24(29(32)33)19-20-26(25)37-38(34,35)36-22-21-30(2,3)4;/h19-20,23H,5-18,21-22H2,1-4H3,(H-,28,31,34,35);1H2
• InChI key: CDTKXKMMWVIQDN-UHFFFAOYSA-N

Description

General description

2-N-Hexadecanoylamino-4-nitrophenylphosphorylcholineis a synthetic chromogenic analog of sphingomyelin.

Application

2-N-Hexadecanoylamino-4-nitrophenylphosphorylcholinemaybe used to evaluate sphingomyelinase activity. It is also suitable forselective assay of brain sphingomyelinase without intrusion from neutralsphingomyelinase.

Warning

Toxicity: Standard Handling (A)

Preparation Note

Slight warming is required for complete solubilization.

Reconstitution

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.

Other Notes

Levade, T., et al. 1983. J. Neurochem.40, 1762.
Gal, A.E., and Fash, F.J. 1976. Chem. Phys. Lipids16, 71.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable