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444143

Sigma-Aldrich

MDM2 Antagonist, Nutlin-3, Racemic

The MDM2 Antagonist, Nutlin-3, Racemic, also referenced under CAS 548472-68-0, controls the biological activity of MDM2. This small molecule/inhibitor is primarily used for Cancer applications.

Synonym(s):

MDM2 Antagonist, Nutlin-3, Racemic, MDM2 Inhibitor IV

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About This Item

Empirical Formula (Hill Notation):
C30H30Cl2N4O4
CAS Number:
548472-68-0
Molecular Weight:
581.49
MDL number:
MFCD07784509
UNSPSC Code:
12352200
NACRES:
NA.77

Quality Level

100

Assay

≥98% (TLC and HPLC)

form

solid

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
protect from light

solubility

DMSO: 25 mg/mL
ethanol: 25 mg/mL

shipped in

wet ice

storage temp.

−20°C

InChI

1S/C30H30Cl2N4O4/c1-18(2)40-25-16-23(39-3)12-13-24(25)29-34-27(19-4-8-21(31)9-5-19)28(20-6-10-22(32)11-7-20)36(29)30(38)35-15-14-33-26(37)17-35/h4-13,16,18,27-28H,14-15,17H2,1-3H3,(H,33,37)

InChI key

BDUHCSBCVGXTJM-UHFFFAOYSA-N

General description

A cell-permeable cis-imidazoline compound that acts as a potent and selective MDM2 antagonist (IC50 = 90 nM for Nutlin-3a and 13.6 µM for Nutlin-3b) and displays antitumor properties. Activates p53 pathway by binding MDM2 in the p53-binding pocket and inhibits MDM2-p53 interaction. Shown to suppress tumor growth by induction of apoptosis in several cancer cells with wild-type p53 and in xenografted tumor mice. A 10 mM (1 mg/172 µl) solution of MDM2 Antagonist, Nutlin-3, Racemic (Cat. No. 444151) in DMSO is also available.
A cell-permeable, potent, and selective MDM2 antagonist (IC50 = 90 nM for Nutlin-3a and 13.6 µM for Nutlin-3b) that displays antitumor properties. Activates the p53 pathway by binding MDM2 in the p53-binding pocket and inhibiting the MDM2-p53 interaction. Shown to induce apoptosis in several cancer cell lines with wild-type p53 and in xenografted tumor mice.

Biochem/physiol Actions

Cell permeable: yes
Primary Target
MDM2
Product does not compete with ATP.
Reversible: no
Target IC50: 90 nM for Nutlin-3a and 13.6 µM for Nutlin-3b

Packaging

Packaged under inert gas

Warning

Toxicity: Harmful (C)

Reconstitution

Following reconstitution, aliquot, purge with inert gas, and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.

Other Notes

Thompson, T., et al. 2004. J. Biol. Chem.279, 53015.
Vassilev, L.T., et al. 2004. Science303, 844.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

444143-1.1ML:
444143-1MG:
444143-MG:

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Tyler P Martinez et al.
eNeuro, 11(3) (2024-02-09)
Alzheimer's Disease (AD) is associated with brain accumulation of synaptotoxic amyloid-β (Aβ) peptides produced by the proteolytic processing of amyloid precursor protein (APP). Cognitive impairments associated with AD correlate with dendritic spine and excitatory synapse loss, particularly within the hippocampus.
Lyubomir T Vassilev et al.
Science (New York, N.Y.), 303(5659), 844-848 (2004-01-06)
MDM2 binds the p53 tumor suppressor protein with high affinity and negatively modulates its transcriptional activity and stability. Overexpression of MDM2, found in many human tumors, effectively impairs p53 function. Inhibition of MDM2-p53 interaction can stabilize p53 and may offer
Thelma Thompson et al.
The Journal of biological chemistry, 279(51), 53015-53022 (2004-10-09)
The p53 tumor suppressor is a key mediator of the cellular response to stress. Phosphorylation induced by multiple stress-activated kinases has been proposed to be essential for p53 stabilization, interaction with transcriptional co-activators, and activation of p53 target genes. However
Ramkrishna Mitra et al.
eLife, 11 (2022-06-14)
Quantification of gene dependency across hundreds of cell lines using genome-scale CRISPR screens has revealed co-essential pathways/modules and critical functions of uncharacterized genes. In contrast to protein-coding genes, robust CRISPR-based loss-of-function screens are lacking for long noncoding RNAs (lncRNAs), which

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