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427211

Sigma-Aldrich

Lactate Dehydrogenase, Porcine Heart

Synonym(s):

Lactate Dehydrogenase, Porcine Heart

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About This Item

CAS Number:
9001-60-9
Enzyme Commission number:
1.1.1.27 (BRENDA, IUBMB)
MDL number:
MFCD00131466
UNSPSC Code:
12352202
NACRES:
NA.54

form

lyophilized

Quality Level

100

specific activity

≥300 units/mg protein

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
desiccated

impurities

≤0.01% PK, MK
≤0.03% GPT, GOT
≤0.1% MDH

pI 

4.6

solubility

aqueous buffer: soluble

shipped in

ambient

storage temp.

−20°C

General description

Native lactate dehydrogenase from porcine heart. A tetrameric enzyme that catalyzes the reversible conversion of pyruvate to lactate. The overall equilibrium of this reaction favors lactate formation. Has an optimal pH of 7.0 and a pI of 4.6. Note: 1KU = 1000 units.
Native lactate dehydrogenase from porcine heart. A tetrameric enzyme that catalyzes the reversible conversion of pyruvate to lactate. The overall equilibrium of this reaction favors lactate formation. Note: 1KU = 1000 units.
Note: 1 KU = 1000 units

Warning

Toxicity: Standard Handling (A)

Unit Definition

One unit is defined as the amount of enzyme that will cause the oxidation of one micromole of NADH per minute at 25°C, pH 7.4
One unit is defined as the amount of enzyme that will oxidize 1.0 µmol NADH per min at 25°C, pH 7.4.

Reconstitution

Following reconstitution, refrigerate (4°C). Stock solutions are stable for up to 24 h at 4°C. Do not freeze solutions.

Other Notes

Kornberg, A. 1995. Methods Enzymol.1, 441

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Pictograms

Health hazard
GHS08

Signal Word

Danger

Hazard Statements

H334

Precautionary Statements

P261 - P284 - P501

Hazard Classifications

Resp. Sens. 1

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

427211-50KU:

Certificates of Analysis (COA)

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