Skip to Content
Merck
All Photos(1)

Documents

Safety Information

407140

Sigma-Aldrich

Anti-IP₃ Receptor Mouse mAb (IPR.1)

liquid, clone IPR.1, Calbiochem®

Sign Into View Organizational & Contract Pricing
All Photos(1)

About This Item

UNSPSC Code:
12352203

biological source

mouse

Quality Level

100

antibody form

purified antibody

antibody product type

primary antibodies

clone

IPR.1, monoclonal

form

liquid

contains

≤0.1% sodium azide as preservative

species reactivity

rabbit, human, canine, mouse, rat, bovine, porcine

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
avoid repeated freeze/thaw cycles

isotype

IgG2b

shipped in

wet ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... ITPR1(3708)
mouse ... Itpr1(16438)
rat ... Itpr1(25262)

General description

Purified mouse monoclonal antibody. Recognizes all of the ~260 kDa IP3 receptor subtypes.
Recognizes all of the ~260 kDa IP3 receptor subtypes.
This Anti-IP₃ Receptor Mouse mAb (IPR.1) is validated for use in ELISA, FC, IF, IHC, IP, Radioimmunoassay, Immunoblotting for the detection of IP₃ Receptor.

Immunogen

a synthetic peptide (GGVGDVLRKPS) corresponding to amino acids near the C-terminus of the IP₃ receptor

Application

ELISA (1:1000)

Flow Cytometry (1:500)

Immunofluorescence (1:100)

Immunohistochemistry (1:100; see application references)

Immunoprecipitation (1:100; see application references)

Radioimmunoassay (1:5000)

Immunoblotting (see application references)

Packaging

Please refer to vial label for lot-specific concentration.

Warning

Toxicity: Standard Handling (A)

Physical form

In 150 mM NaCl, 10 mM sodium phosphate, pH 7.5.

Reconstitution

Following initial thaw, aliquot and freeze (-70°C).

Analysis Note

Positive Control
Lymphocytes, macrophages, granulocytes, fibroblasts, epithelial and edothelial cells, skeletal muscle, cardiac muscle and brain tissue

Other Notes

Bourguignon, L.Y.W., et al. 1993. Cell Biol. Int.17, 751.
Bourguignon, L.Y.W., et al. 1993. J. Biol. Chem.268, 7290.
Specific for the C-terminal cytoplasmic domain of the IP3 receptor. For immunohistochemistry, this antibody requires FITC and confocal microscopy. Variables associated with assay conditions will dictate the proper working dilution.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Not finding the right product?  

Try our Product Selector Tool.

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

407140-50UG:
407140-UG:

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

L Cavallini et al.
The Journal of biological chemistry, 271(10), 5545-5551 (1996-03-08)
Prostaglandin I2 (PGI2) and sodium nitroprusside (SNP) induce a rapid decay of the thrombin-promoted increase of [Ca2+]i in aspirin-treated platelets incubated in the absence of external Ca2+. The mechanism of their effect was studied with a new method which utilizes
John C Hennessey et al.
Pharmacology research & perspectives, 3(2), e00112-e00112 (2015-03-03)
Endothelial cell (EC)-dependent vasodilation by proteinase-activated receptor 2 (PAR2) is preserved in small caliber arteries in disease states where vasodilation by muscarinic receptors is decreased. In this study, we identified and characterized the PAR2-mediated intracellular calcium (Ca(2+))-release mechanisms in EC
Xudong Feng et al.
Nature communications, 5, 4487-4487 (2014-07-30)
Inositol 1, 4, 5-trisphosphate receptor (IP3R)-mediated Ca(2+) release from the endoplasmic reticulum (ER) triggers many physiological responses in neurons, and when uncontrolled can cause ER stress that contributes to neurological disease. Here we show that the unfolded protein response (UPR)
L Lagostena et al.
The Journal of physiology, 531(Pt 3), 693-706 (2001-03-17)
1. Hensen's cells in the isolated cochlea were stimulated by extracellular adenosine 5'-triphosphate (ATP) applied to their endolymphatic surface while changes in membrane current and intracellular calcium concentration ([Ca2+]i) were measured simultaneously. The response consisted of (i) an initial rapid
Chenxi Guo et al.
Journal of cell science, 133(13) (2020-06-18)
The role of two-pore channel type 2 (TPC2, encoded by tcpn2)-mediated Ca2+ release was recently characterized in zebrafish during establishment of the early spinal circuitry, one of the key events in the coordination of neuromuscular activity. Here, we extend our
View All Related Papers

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service