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405210

Sigma-Aldrich

ID-8

A cell-permeable indole compound that, when added to murine D3ES ESC (embryonic stem cell) cultures in the absence of serum and MEF (murine embryonic fibroblast) feeder cells, serves as a cheaper, chemical alternative to LIF

Synonym(s):

ID-8, 1-(4-Methoxyphenyl)-2-methyl-3-nitro-indol-6-ol

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About This Item

Empirical Formula (Hill Notation):
C16H14N2O4
CAS Number:
147591-46-6
Molecular Weight:
298.29
UNSPSC Code:
12352200
NACRES:
NA.51

Quality Level

100

Assay

≥95% (HPLC)

form

powder

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
protect from light

color

yellow

solubility

DMSO: 50 mg/mL

shipped in

ambient

storage temp.

2-8°C

General description

A cell-permeable indole compound that, when added (10 µM) to murine D3ES ESC (embryonic stem cell) cultures in the absence of serum and MEF (murine embryonic fibroblast) feeder cells, serves as a cheaper, chemical alternative to LIF (1,000 units/ml) for expanding ESCs in their undifferentiated state. Long-term murine ESCs maintained under ID-8 are capable of differentiating and EBs (Embryoid Bodies) formation upon ID-8 removal. Also claimed to maintain human ESCs in their pluripotent state in the absence of MEF.

Packaging

Packaged under inert gas

Warning

Toxicity: Standard Handling (A)

Other Notes

Miyabayashi, T., et al. 2008. Biosci. Biotechnol. Biochem.72, 1242.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

405210-MG:
405210-10MG:

Certificates of Analysis (COA)

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Tomoyuki Miyabayashi et al.
Bioscience, biotechnology, and biochemistry, 72(5), 1242-1248 (2008-05-08)
Embryonic stem cells (ESCs), which have characteristics such as self-renewal, indefinite proliferation, and pluripotency, are thought to hold great promise for regenerative medicine. ESCs are generally cultured on mouse embryonic fibroblast (MEF) or MEF conditioned medium (MEF-CM). However, for therapeutic

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