341599

FGF-10, Human, Recombinant

• UNSPSC Code: 12352202

Properties

• Assay: ≥97% (SDS-PAGE)
• Quality Level: 100
• form: liquid
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze
• impurities: <1.0 EU/μg protein Endotoxin
• shipped in: ambient
• storage temp.: −20°C

Description

General description

Recombinant, human fibroblast growth factor 10 (FGF-10) consisting of amino acids 137-208 and expressed in E. Coli. Mature human FGF-10, a ~20 kDa protein, contains a serine-rich region near its N-terminus sharing 93% amino acid sequence identity with mouse FGF-10 and 96% with rat FGF-10.

Biochem/physiol Actions

ED₅₀ = 20-100 ng/ml measured in a cell proliferation assay using 4MBr-5 monkey epithelial cells.

Warning

Toxicity: Standard Handling (A)

Physical form

Lyophilized from 0.2 µm sterile-filtered MOPS, Na₂SO₄, EDTA, DTT, BSA.

Reconstitution

Following reconstitution refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C) for long-term storage. Stock solutions are stable for up to 1 month at 4°C or for up to 3 months at -20°C.

Reconstitute to 100 µg/ml in sterile PBS containing at least 0.1% human or bovine serum albumin.

Other Notes

Beenken, A. and M. Mohammadi. 2009. Nat. Rev. Drug Discov.8, 235.
Nomura, S., et al. 2008. Br. J. Cancer99, 305.
Zhang, X., et al. 2006. J. Biol. Chem.281, 15694.
Beer, H.D., et al. 2005. Oncogene24, 5269.
Jaskoll, T., et al. 2005. BMC Dev. Biol.5, 11.
Rice, R., et al. 2004. J. Clin. Invest.113, 1692.
Donjacour, A.A., et al. 2003. Dev. Biol.261, 39.
Mailleux, A.A., et al. 2002. Development129, 53.
Sakaue, H., et al. 2002. Genes Dev.16, 908.
Makarenkova, H.P., et al. 2000. Development127, 2563.
Makarenkova, H.P., et al. 2000. Development127, 2563.
Pirvola, U., et al. 2000. J. Neurosci.20, 6125.
Weaver, M., et al. 2000. Development127, 2695.
Igarashi, M., et al. 1998. J. Biol. Chem.273, 13230.
Min, H., et al. 1998. Genes Dev.12, 3156.
Bellusci, S., et al. 1997. Development124, 4867.
Emoto, H., et al. 1997. J. Biol. Chem.272, 23191.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3