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23-036-M

Sigma-Aldrich

UbcH6 Protein, active, 10 µg

Active, full length, recombinant human UbcH6. N-terminal HA, 6His-tagged. For use in Enzyme Assays. Functions as an E2 enzyme in ubiquitination assays.

Synonym(s):

Ubiquitin-conjugating enzyme E2 E1, Ubiquitin-protein ligase E1, UBE2E1

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About This Item

UNSPSC Code:
12352202
eCl@ss:
32160405
NACRES:
NA.77

biological source

human

Quality Level

100
300

manufacturer/tradename

Upstate®

technique(s)

activity assay: suitable

NCBI accession no.

NM_003341

UniProt accession no.

P51965

General description

Ubiquitin-conjugating (E2) enzymes are characterized by the presence of a highly conserved ubiquitin-conjugating domain which accommodates ATP-activated ubiquitin (Ub) via a covalently linked thioester onto its active-site residue. E2 enzymes act via selective protein-protein interactions with the ubiquitin-activating E1 enzyme and ubiquitin ligase E3 enzymes and are able to differentiate effects on downstream substrates, either with a single Ub molecule or a Ub chain. While E3s are involved in substrate selection, E2s are the main determinants for selection of the lysine to construct Ub chains, which thereby directly control the cellular fate of the substrate.
UbcH6 has been shown to interact with a number of diverse E3 ligases, including RNF20/40, Trim32 and WWP2.

Application

Active, full length, recombinant human UbcH6. N-terminal HA, 6His-tagged. For use in Enzyme Assays. Functions as an E2 enzyme in ubiquitination assays.
Ubiquitination Cascade Comoponent: E2

Physical form

Purified using immobilized metal affinity chromatography.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Pictograms

Exclamation mark
GHS07

Signal Word

Warning

Hazard Statements

H317

Hazard Classifications

Skin Sens. 1

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

PDSCL

Deleterious substance

JAN Code

23-036:
23-036M:

Certificates of Analysis (COA)

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