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208773

Sigma-Aldrich

Calpain Substrate IV, Cell-Permeable, Fluorogenic

The non-permeant fluorogenic Calpain Substrate III is made cell-permeable by a c-terminal heptaarginine extension via a Glu linkage.

Synonym(s):

Calpain Substrate IV, Cell-Permeable, Fluorogenic

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About This Item

Empirical Formula (Hill Notation):
C127H206N50O27S
Molecular Weight:
2897.38
UNSPSC Code:
12352200
NACRES:
NA.77

Quality Level

100

Assay

≥95% (HPLC)

form

lyophilized solid

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
desiccated (hygroscopic)
protect from light

solubility

DMSO: 5 mg/mL

fluorescence

λex 480 nm; λem 320 nm

shipped in

ambient

storage temp.

−20°C

General description

The non-permeant fluorogenic Calpain Substrate III (Cat. No. 208771) is made cell-permeable by a c-terminal heptaarginine extension via a Glu linkage. In addition to the expected improvement in permeability, the modified peptide also exhibits enhanced Calpain B sensitivity (kcat = 0.2 s-1, kcat/KM = 5000 M-1 s-1) when compared to the parental peptide (kcat = 0.17 s-1, kcat/KM = 680 M-1 s-1). Its suitability in detecting cellular Ca2+-dependent calpain activity is demonstrated both in intact Drosophila melanogaster S2 cells following ionomycin stimulation and in S2 lysates via direct Ca2+ addition.
The non-permeant fluorogenic Calpain Substrate III (Cat. No. 208771) is made cell-permeable by a c-terminal heptaarginine extension via a Glu linkage. In addition to the expected improvement in permeability, the modified peptide also exhibits enhanced Calpain B sensitivity (kcat = 0.2 s-1, kcat/KM = 5000 M-1 s-1) when compared to the parental peptide (kcat = 0.17 s-1, kcat/KM = 680 M-1 s-1). Its suitability in detecting cellular Ca2+-dependent calpain activity is demonstrated both in intact Drosophila melanogaster S2 cells following ionomycin stimulation and in S2 lysates via direct Ca2+ addition.
The non-permeant fluorogenic Calpain Substrate III is made cell-permeable by a c-terminal heptaarginine extension via a Glu linkage.

Packaging

Packaged under inert gas

Warning

Toxicity: Standard Handling (A)

Sequence

(DABCYL)-Thr-Pro-Leu-Lys~Ser-Pro-Pro-Pro-Ser-Pro-Arg-Glu(EDANS)-Arg-Arg-Arg-Arg-Arg-Arg-Arg-NH₂

Physical form

Supplied as a trifluoroacetate salt

Reconstitution

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.

Other Notes

Banoczi, Z., et al. 2008. Bioconjug. Chem.19, 1375.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

208773-1MG:
208773-MG:

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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