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17-128

Sigma-Aldrich

Alkaline/Acid Phosphatase Assay Kit (R-R-A-pS-V-A)

Alkaline/Acid Phosphatase Assay Kit is routinely used to detect phosphatase activity by either dephosphorylation of the phosphopeptide (RRApSVA) or hydrolysis of pNPP.

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About This Item

UNSPSC Code:
12161503
eCl@ss:
32161000
NACRES:
NA.84

Quality Level

100

manufacturer/tradename

Upstate®

technique(s)

activity assay: suitable (phosphatase)

detection method

colorimetric

shipped in

wet ice

Application

Used to detect/quantify: Alkaline/Acid

Packaging

Kit capacity: 100 assays

Components

Malachite Green Solution A (Cat.# 20-105)

Malachite Green Additive (Cat.# 20-104)

Phosphate Standard (Cat.# 20-103)

Serine Phosphopeptide (RRApSVA) (Cat.# 12-220)

pNPP (p-Nitrophenyl Phosphate) (Cat.# 20-106)

NiCl2, 40mM (Cat.# 20-178)

pNPP Ser/Thr Assay Buffer (Cat.# 20-179)

96-well microtiter plate

Quality

routinely used to detect phosphatase activity by either dephosphorylation of the phosphopeptide (RRApSVA) or hydrolysis of pNPP

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Signal Word

Danger

Hazard Classifications

Aquatic Chronic 3 - Carc. 1A Inhalation - Met. Corr. 1 - Repr. 1B - Skin Sens. 1 - STOT RE 2

Target Organs

Lungs

Storage Class Code

6.1D - Non-combustible acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects

WGK

WGK 3

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

17-128:

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Quantification of subnanomolar amounts of phosphate bound to seryl and threonyl residues in phosphoproteins using alkaline hydrolysis and malachite green.
Ekman, P and Jager, O
Analytical biochemistry, 214, 138-141 (1993)
An investigation of the substrate specificity of protein phosphatase 2C using synthetic peptide substrates; comparison with protein phosphatase 2A
Donella Deana, A., et al
Biochimica et Biophysica Acta, 1051, 199-202 (1990)
Nathan J Castro et al.
Tissue engineering. Part A, 22(13-14), 940-948 (2016-06-15)
Osseous tissue defects caused by trauma present a common clinical problem. Although traditional clinical procedures have been successfully employed, several limitations persist with regards to insufficient donor tissue, disease transmission, and inadequate host-implant integration. Therefore, this work aims to address
P P Van Veldhoven et al.
Analytical biochemistry, 161(1), 45-48 (1987-02-15)
A procedure, based on the complex formation of malachite green with phosphomolybdate under acidic conditions, to measure inorganic orthophosphate in the nanomolar range is described. The addition of polyvinyl alcohol is required to stabilize the dye-phosphomolybdate complex. The advantages of
A Donella-Deana et al.
Biochimica et biophysica acta, 1094(1), 130-133 (1991-08-13)
The four main classes of protein phosphatases (PP-1, 2A, 2B and 2C), although differing in their ability to dephosphorylate phosphopeptide substrates, invariably display a marked preference toward phosphothreonyl peptides over their phosphoseryl counterparts. Conversely, all the acidic and alkaline phosphatases
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