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Safety Information

16-173

Sigma-Aldrich

Anti-FAK Antibody, clone 4.47, agarose conjugate

clone 4.47, Upstate®, from mouse

Synonym(s):

Anti-FADK, Anti-FADK 1, Anti-FAK, Anti-FAK1, Anti-FRNK, Anti-PPP1R71, Anti-p125FAK, Anti-pp125FAK

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

100

antibody product type

primary antibodies

clone

4.47, monoclonal

species reactivity

human

manufacturer/tradename

Upstate®

technique(s)

immunoprecipitation (IP): suitable

NCBI accession no.

NM_005607.3
NM_153831.2

UniProt accession no.

Q05397

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... PTK2(5747)

General description

Anti-FAK monoclonal antibody (05-537), covalently coupled to Protein G agarose by dimethyl-pimelimidate

Application

Research Category
Signaling
Research Sub Category
Cytoskeletal Signaling
Suitable for immunoprecipitation and affinity purification.
This Anti-FAK Antibody, clone 4.47, agarose conjugate is validated for use in IP for the detection of FAK.

Features and Benefits

Format: Gel Immobilized

Quality

routinely evaluated by immunoprecipitating FAK from a mouse 3T3/A31 RIPA cell lysate, as confirmed by subsequent immunoblot analysis using anti-FAK (05-537)

Physical form

PBS containing 0.05% sodium azide

Storage and Stability

2 years at 4°C

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

16-173:

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Eugene Ciccimaro et al.
Analytical chemistry, 81(9), 3304-3313 (2009-04-10)
A vital point of convergence for many signaling pathways at cellular focal adhesions is the interaction of two nonreceptor tyrosine kinases, focal adhesion kinase (FAK) and Src. The binding of Src to FAK leads to the phosphorylation of Y(576) and
Attila Aszodi et al.
Genes & development, 17(19), 2465-2479 (2003-10-03)
Beta1 integrins are highly expressed on chondrocytes, where they mediate adhesion to cartilage matrix proteins. To assess the functions of beta1 integrin during skeletogenesis, we inactivated the beta1 integrin gene in chondrocytes. We show here that these mutant mice develop
Eugene Ciccimaro et al.
Molecular pharmacology, 75(3), 658-666 (2008-12-23)
A recently developed stable isotope dilution liquid chromatography-multiple reaction/mass spectrometry method to quantify focal adhesion kinase (FAK) activation loop phosphorylation was used to study endogenous Src kinase activity. This revealed that bis-phosphorylated pTyr(576)/Tyr(577)-FAK was a biomarker of Src activity and
Gabriela D'Amico et al.
Development (Cambridge, England), 149(13) (2022-06-21)
Precise vascular patterning is crucial for normal growth and development. The ERG transcription factor drives Delta-like ligand 4 (DLL4)/Notch signalling and is thought to act as a pivotal regulator of endothelial cell (EC) dynamics and developmental angiogenesis. However, molecular regulation
Elena Kurenova et al.
Cell cycle (Georgetown, Tex.), 13(16), 2542-2553 (2014-12-09)
Melanoma has the highest mortality rate of all skin cancers and a major cause of treatment failure is drug resistance. Tumors heterogeneity requires novel therapeutic strategies and new drugs targeting multiple pathways. One of the new approaches is targeting the

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