14-760-M

Ret (V804M) Protein, active, 10 µg

Active, N-Terminal GST-tagged, recombinant, human Ret amino acids 658 – end containing the V804M mutation. For use in Kinase Assays.

• UNSPSC Code: 12352202
• eCl@ss: 32160405
• NACRES: NA.32

Properties

• biological source: human
• Quality Level: 100; 300
• recombinant: expressed in baculovirus infected Sf21 cells
• mol wt: M_w 79.2 kDa
• manufacturer/tradename: Upstate^®
• technique(s): activity assay: suitable (kinase)
• NCBI accession no.: NM_000323
• UniProt accession no.: P07949; P35546 (mouse)
• Gene Information: human ... RET(5979)

Description

General description

N-Terminal GST-tagged, recombinant, human Ret amino acids 658 – end containing the V804M mutation.

Product Source: Expressed by baculovirus in Sf21 insect cells.

The V804M mutation has been identified in patients with familial medullary thyroid carcinoma (FMTC) (Fink et al, (1996), Int. J. Cancer, 69, 312-316; Lesueur F. et al,(2005), JCEM, 90, 3454-3457).

Application

Research Category
Inflammation & Immunology

Biochem/physiol Actions

Protein Target: Ret

Target Sub-Family: TK

Packaging

Also available in 250 µg size --call for pricing and availability and reference catalog number 14-760M when ordering the 250 µg size.

Quality

Routinely evaluated by phosphorylation of CHKtide.

Physical form

50mM Tris-HCl pH7.5, 300mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.

Glutathione agarose affinity chromatography

Storage and Stability

Store -70°C. For maximum recovery of product, centrifuge original vial prior to removing the cap.
Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into pre-chilled microcentrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70°C.

Other Notes

For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Pictograms: GHS07
• Signal Word: Warning
• Hazard Statements: H317
• Precautionary Statements: P261 - P272 - P280 - P302 + P352 - P333 + P313 - P362 + P364
• Hazard Classifications: Skin Sens. 1
• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable