14-276-M

Akt1/PKBα Protein, active, 15 µg

Active, recombinant full-length human Akt1, containing N-terminal His6 tag, for use in IP-Kinase Assays & Kinase Assays.

• Synonym(s): Protein kinase B alpha, Proto-oncogene c-Akt, RAC, RAC-PK-alpha
• UNSPSC Code: 12352202
• eCl@ss: 32160405
• NACRES: NA.26

Properties

• biological source: human
• Quality Level: 100
• recombinant: expressed in baculovirus infected Sf21 cells
• form: liquid
• shelf life: 1 yr
• mol wt: M_w 59.9 kDa
• manufacturer/tradename: Upstate^®
• technique(s): activity assay: suitable (IP-kinase); activity assay: suitable (kinase)
• solubility: soluble
• NCBI accession no.: NM_001014431.1; NM_001014432.1; NM_005163.2
• UniProt accession no.: P31749
• storage temp.: −70°C
• Gene Information: human ... AKT1(207)

Description

General description

Research area: CELL SIGNALING

Product Source: Expressed by baculovirus in Sf21 insect cells

Recombinant full-length human Akt1, containing N-terminal His6-tag.

Biochem/physiol Actions

Protein Target: Akt1/PKBα

Target Sub-Family: AGC

Akt, also known as protein Kinase B (PKB), is a crucial protein involved in various cell signaling pathways. It modifies and controls the activities of numerous proteins involved in a wide range of cellular and physiological processes. Some of these processes include cell survival, apoptosis, cell proliferation, transcription, cell migration, and metabolism. Akt also helps in influencing angiogenesis, and normal development of embryos. Akt regulates cell survival through the phosphorylation of mitogen-activated protein kinase kinase kinase 5 (MAP3K5), which is involved in the cellular apoptotic response. The Akt occupies a pivotal position in the phosphoinositide 3-kinase-protein kinase B-mammalian target of rapamycin (PI3K-PKB-mTOR) signaling pathway. Genetic mutations occurring upstream of Akt in this cascade can result in uncontrolled cell proliferation and the development of cancer. In addition abberent Akt activation is associated with the development of type-2 diabetes.

Packaging

Also available in 250μg size, please inquire for pricing and availability.

Quality

Routinely evaluated by phosphorylation of Crosstide. Please refer to the Certificate of Analysis for more information.

Physical form

Ni²⁺/NTA and glutathione agarose

Supplied in 50mM Tris/HCl pH7.5, 150mM NaCl, 150mM imidazole, 1mM benzamidine, 0.2mM PMSF, 0.1mM EGTA, 0.07% 2-mercaptoethanol, 0.02% Brij 35, 270mM sucrose. Frozen solution.

Storage and Stability

1 year at -70°C

Other Notes

For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Pictograms: GHS08,GHS07
• Signal Word: Danger
• Hazard Statements: H315,H317,H319,H360D
• Precautionary Statements: P202 - P261 - P280 - P302 + P352 - P305 + P351 + P338 - P308 + P313
• Hazard Classifications: Eye Irrit. 2 - Repr. 1B - Skin Irrit. 2 - Skin Sens. 1
• Storage Class Code: 6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
• WGK: WGK 2