Skip to Content
Merck
All Photos(1)

Documents

Safety Information

13-173

Sigma-Aldrich

MBP, recombinant

Synonym(s):

myelin basic protein

Sign Into View Organizational & Contract Pricing
All Photos(1)

About This Item

UNSPSC Code:
12352200
eCl@ss:
32160405
NACRES:
NA.41

biological source

bovine

Quality Level

100

form

liquid

mol wt

Mw ~47 kDa

manufacturer/tradename

Upstate®

concentration

1 mg/mL

technique(s)

activity assay: suitable

NCBI accession no.

NM_001025081.1
NM_001025090.1
NM_001025092.1
NM_001025094.1
NM_001025098.1
NM_001025100.1
NM_001025101.1
NM_002385.2

UniProt accession no.

P02686

shipped in

dry ice

General description

Product Source: Recombinant fusion protein containing bovine myelin basic protein (MBP) with an N-terminal GST tag and a C-terminal 6His tag, expressed in E. coli.

Quality

Routinely evaluated by kinase assay

Physical form

500 µg recombinant MBP in 500 µl of 50mM Tris-HCl, pH 7.5, 150mM NaCl, 500 mM imidazole, 1 mM PMSF, and 10% glycerol. Frozen solution.
ProBond Resin chromatography

Storage and Stability

Stable for 2 years at -20C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Analysis Note

Specific Activity: This lot was successfully phosphorylated in a non-radioactive kinase assay using recombinant active MAP Kinase 2/Erk2 (Catalog # 14-173). MBP phosphorylation was detected by immunoblot analysis using anti-phospho-MBP (Catalog # 05-429). Immunoblot shown to the right.

Other Notes

Purity information is lot specific, please consult the certificate of analysis

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

13-173:

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Vanessa D Stewart et al.
FEBS letters, 596(22), 2952-2973 (2022-09-15)
Myelin-associated glycoprotein (MAG) and Nogo inhibit neurite outgrowth by binding to receptors such as NgR1, PirB and LRP1, and they have also been shown to induce phosphorylation of Smad2, a key intermediate in the transforming growth factor β (TGFβ) signalling

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service