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13-107

Sigma-Aldrich

Core Histones

Highly pure core histones proteins (H2A, H2B, H3 and H4) including H1 purified from chicken erythrocytes, suitable as a substrate for histone modification assays (HAT, HDAC, DNMT) and nucleosome assembly studies.

Synonym(s):

Core Histone Proteins

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About This Item

UNSPSC Code:
12352200
eCl@ss:
32160405
NACRES:
NA.32

biological source

chicken

manufacturer/tradename

Upstate®

technique(s)

activity assay: suitable (histone acetyltransferase (HAT))

shipped in

dry ice

General description

Histones are a family of basic proteins that associate with DNA in the nucleus and help condense it into chromatin. Some histones function as spools for the thread-like DNA to wrap around forming a nucleosome. Each nucleosome is made of DNA wrapped around eight histone proteins that function like a spool and are called a histone octamer. Each histone octamer is composed of two copies each of the histone proteins H2A, H2B, H3, and H4. The chain of nucleosomes is then wrapped into a 30 nm spiral called a solenoid, where additional H1 histone proteins are associated with each nucleosome to maintain the chromosome structure.

Application

Core histones and histone H1, purified from chicken erythrocytes; suitable as a substrate for HAT assays; purity = 90%

Quality

rountinely evaluated by immunoblot using Anti-Histone H3 (05-499) and Anti-Histone H4 (07-108)

Physical form

Lyophilized

Storage and Stability

Lyophilized: 2 years at -20°C; Rehydrated: 6 months at -70°C

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

11 - Combustible Solids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

13-107:

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Analysis of nuclear receptor acetylation.
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M R Fibi,M Aslan,P Hintz-Obertreis,J U Pauly,M Gerken,G Luben,L Lauffer,B Siebold,W Stuber,G Nau
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