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Safety Information

12-457

Sigma-Aldrich

MYPT1 (654-880)

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About This Item

UNSPSC Code:
12352200
eCl@ss:
32160405
NACRES:
NA.41

Assay

≥70% (Coomassie staining, SDS-PAGE)

Quality Level

100

form

liquid

manufacturer/tradename

Upstate®

technique(s)

activity assay: suitable (kinase)

NCBI accession no.

NM_002480.1

UniProt accession no.

O14974

shipped in

dry ice

Gene Information

human ... PPP1R12A(4659)

General description

Product Source: expressed in E. coli
ROK substrate protein corresponding to amino acids 654-880 of human MYPT1 with an N-terminal GST tag. MW = 60 kDa.

Biochem/physiol Actions

Protein Target: myosin phosphatase

Quality

Routinely evaluated as a substrate in a Protein Kinase Assay.

Physical form

100mM Tris-HCl pH 8.0, 200mM NaCl, 10mM glutathione and 5μg/ml leupeptin. Frozen solution.

Storage and Stability

1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery, centrifuge the vial prior to removing the cap.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Pictograms

Health hazard
GHS08

Signal Word

Danger

Hazard Statements

H334

Precautionary Statements

P261 - P284 - P501

Hazard Classifications

Resp. Sens. 1

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

12-457:

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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J Feng et al.
The Journal of biological chemistry, 274(52), 37385-37390 (1999-12-22)
It is clear from several studies that myosin phosphatase (MP) can be inhibited via a pathway that involves RhoA. However, the mechanism of inhibition is not established. These studies were carried out to test the hypothesis that Rho-kinase (Rho-associated kinase)
G Totsukawa et al.
The Journal of cell biology, 144(4), 735-744 (1999-02-26)
It has been demonstrated previously that during mitosis the sites of myosin phosphorylation are switched between the inhibitory sites, Ser 1/2, and the activation sites, Ser 19/Thr 18 (Yamakita, Y., S. Yamashiro, and F. Matsumura. 1994. J. Cell Biol. 124:129-
Yi-Ju Lee et al.
Journal of cellular physiology, 220(2), 476-484 (2009-04-25)
Cellular responses are determined by a number of signaling cues in the local microenvironment, such as growth factors and extracellular matrix (ECM). In cultures of mammary epithelial cells (MECs), functional differentiation requires at least two types of signal, lactogenic hormones

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