1.00415

A-1 Medium

GranuCult^® prime, suitable for microbiology, for coliforms

• Synonym(s): Coliform culture media
• UNSPSC Code: 41106213
• NACRES: NA.74

Properties

• Agency: US EPA 8368
• Quality Level: 200
• form: medium granules (dehydrated (DCM))
• manufacturer/tradename: GranuCult^® prime
• packaging: pkg of -500 g
• storage condition: dry media (Tightly closed); protect from light
• technique(s): microbiological culture: suitable
• color: light brown
• pH: 6.7-7.1 (25 °C, 31.5 g/L in H₂O)
• application(s): food and beverages; microbiology
• storage temp.: 15-25°C
• suitability: coliforms

Description

General description

A-1 Medium is a granulated dehydrated culture medium used to detect fecal coliforms in non-potable and wastewater. Peptone from casein, lactose, and salicin are nutrients and guarantee good growth of microorganisms. Sodium chloride provides osmotic balance. Triton^™ X 100 is incorporated as a detergent.

Application

A-1 Medium is a selective culture medium for the detection of fecal coliforms in non-potable and wastewater.

Features and Benefits

• GranuCult^® prime offers superior granulated culture media
• Safe and sustainable due to reduced risks associated with fine dust and toxic substance inhalation, resulting in a safer work environment
• Excellent wettability, solubility, and free flowing properties
• Convenient, with minimal component separation and clumping, even under warm or humid conditions
• High batch-to-batch reproducibility
• Prolonged shelf life of up to five years
• High number of test strains exceeding all regulatory demands
• Granulation technology allowing many supplements to be included, with no need to add these separately

Analysis Note

Appearance (clearness): clear
Appearance (colour): yellowish to yellow
pH-value (25 °C): 6.7 - 7.1
Growth (Escherichia coli ATCC 25922 (WDCM 00013)): good to very good
Growth (Escherichia coli ATCC 8739 (WDCM 00012)): good to very good
Growth (Enterobacter aerogenes ATCC 13048): none to fair
Growth (Enterococcus faecalis ATCC 19433 (WDCM 00009)): none to fair
Growth (Bacillus subtilis ATCC 6633 (WDCM 00003)): none
Growth (Aeromonas hydrophila ATCC 7966 (WDCM 00063)): none
Gas (Escherichia coli ATCC 25922 (WDCM 00013)): +
Gas (Escherichia coli ATCC 8739 (WDCM 00012)): +
Gas (Enterobacter aerogenes ATCC 13048): none or poor
Gas (Enterococcus faecalis ATCC 19433 (WDCM 00009)): -
Gas (Bacillus subtilis ATCC 6633 (WDCM 00003)): -
Gas (Aeromonas hydrophila ATCC 7966 (WDCM 00063)): -
Incubation: 3 hrs.; 35 °C; aerobic. 19 - 23 hrs.; 44.5 °C.

Footnote

We offer two media types: the superior granulated GranuCult^® prime and the cost-efficient powdered NutriSelect^® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany

NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow

Safety Information

• Pictograms: GHS05,GHS07,GHS09
• Signal Word: Danger
• Hazard Statements: H317 - H318 - H410
• Precautionary Statements: P261 - P272 - P273 - P280 - P302 + P352 - P305 + P351 + P338
• Hazard Classifications: Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Skin Sens. 1
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3