09-813
Anti-RING finger protein 8 Antibody
from rabbit, purified by affinity chromatography
Synonym(s):
ring finger protein 8, C3HC4-type zinc finger protein, ring finger protein (C3HC4 type) 8, UBC13/UEV-interacting ring finger protein, E3 ubiquitin-protein ligase RNF8
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All Photos(3)
About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Pricing and Inventory is not available. Please check the Merck website for more information.
biological source
rabbit
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
purified by
affinity chromatography
species reactivity
human, mouse
species reactivity (predicted by homology)
chimpanzee (based on 100% sequence homology), rhesus macaque (based on 100% sequence homology)
technique(s)
immunocytochemistry: suitable
western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... RNF8(9025)
General description
RING Finger protein 8 (RNF8) is DNA binding protein belonging to the RING (Really Interesting New Gene) finger group of proteins. This family is characterized by a RING finger domain containing a Cys3HisCys4 amino acid motif capable of binding two zinc cations. RNF8 contains two domains of interest, the characteristic RING finger motif in the C-terminus and a FHA domain within the N-terminus, and is a critical E3 ubiquitin-protein ligase involved in the assembly of repair proteins to damaged DNA sites. After DNA double-strand breaks, RNF8 is recruited by ATM-phosphorylated MDC1 to the damage sites, where it functions to mediate H2A and H2AX ubiquitination, which in turn promotes TPR53BPI formation and the ionizing radiation-induced foci (IRIF) of BRCA1. RNF8 may also play regulatory roles in cytokinesis, correct exiting from mitosis, and RXRA-mediated transcriptional activity.
Specificity
This antibody recognizes RING finger protein 8.
Immunogen
Linear peptide corresponding to amino acids 135-148 of human RING finger protein 8.
Application
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Histone Modifying Proteins
Cell Cycle, DNA Replication & Repair
Histone Modifying Proteins
Cell Cycle, DNA Replication & Repair
Use Anti-RING finger protein 8 Antibody (Rabbit Polyclonal Antibody) validated in WB, ICC to detect RING finger protein 8 also known as C3HC4-type zinc finger protein.
Western Blot (SNAP ID) Analysis: 1 µg/mL of a representative lot of this antibody detected RING finger protein 8 on 10 µg of U2OS nuclear extract.
Immunocytochemistry Analysis: 1:500 dilution of a representative lot this antibody detected RING finger protein 8 in NIH/3T3 and A431 cells.
Immunocytochemistry Analysis: 1:500 dilution of a representative lot this antibody detected RING finger protein 8 in NIH/3T3 and A431 cells.
Quality
Evaluated by Western Blot in U2OS nuclear extract.
Western Blot Analysis: 0.1 µg/mL of this antibody detected RING finger protein 8 on 10 µg of U2OS nuclear extract.
Western Blot Analysis: 0.1 µg/mL of this antibody detected RING finger protein 8 on 10 µg of U2OS nuclear extract.
Target description
~ 58 kDa observed. Two different isoforms have been described. Isoform 1: 55.5 kDa and Isoform 2: 11 kDa
Physical form
Affinity purified
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Control
U2OS nuclear extract
U2OS nuclear extract
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Regulatory Listings
Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.
JAN Code
09-813:
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Feng Zhao et al.
Oncology letters, 21(1), 42-42 (2020-12-03)
Breast cancer is one of the malignant tumors with the highest mortality rate. With the development of precise treatment technology for cancer, numerous molecular targets have been identified and applied in the treatment of diseases. The present study investigated the
John J Krais et al.
Cancer research, 80(13), 2848-2860 (2020-03-28)
BRCA1 gene mutations impair homologous recombination (HR) DNA repair, resulting in cellular senescence and embryonic lethality in mice. Therefore, BRCA1-deficient cancers require adaptations that prevent excessive genomic alterations from triggering cell death. RNF168-mediated ubiquitination of γH2AX at K13/15 (ub-H2AX) serves
Tingting Zhou et al.
Cell death & disease, 13(4), 352-352 (2022-04-17)
Androgen receptor (AR) signaling drives prostate cancer (PC) progression. Androgen deprivation therapy (ADT) is temporally effective, whereas drug resistance inevitably develops. Abnormal expression of AR/ARV7 (the most common AR splicing variant) is critical for endocrine resistance, while the detailed mechanism
Somaira Nowsheen et al.
Nature communications, 9(1), 2736-2736 (2018-07-18)
Cells respond to cytotoxic DNA double-strand breaks by recruiting repair proteins to the damaged site. Phosphorylation of the histone variant H2AX at S139 and Y142 modulate its interaction with downstream DNA repair proteins and their recruitment to DNA lesions. Here
Qinglei Hang et al.
Nature communications, 12(1), 4033-4033 (2021-07-01)
In response to DNA double-strand breaks (DSBs), repair proteins are recruited to the damaged sites. Ubiquitin signaling plays a critical role in coordinating protein recruitment during the DNA damage response. Here, we find that the microRNA biogenesis factor DGCR8 promotes
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