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07-889

Sigma-Aldrich

Anti-phospho-PTEN (Ser370) Antibody

Upstate®, from rabbit

Synonym(s):

MMAC1, TEP1, mutated in multiple advanced cancers 1

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

100

antibody form

affinity purified immunoglobulin

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

mouse

manufacturer/tradename

Upstate®

technique(s)

western blot: suitable

isotype

IgG

NCBI accession no.

NM_000314.4

UniProt accession no.

P60484

shipped in

wet ice

target post-translational modification

phosphorylation (pSer370)

Gene Information

human ... PTEN(5728) , TEP1(7011)

Specificity

Predicted to cross-react with human and rat based on 100 % sequence homology, but have not been tested.
Recognizes phosphorylated PTEN (Ser370)

Immunogen

Peptide corresponding to amino acid region encompassing the human, mouse, and rat phospho-PTEN (Ser370)

Application

Anti-phospho-PTEN (Ser370) Antibody is an antibody against phospho-PTEN (Ser370) for use in WB.
Immunoblot Analysis: A 1:1000 dilution of this lot detected phospho-PTEN (Ser370) in RIPA lysates of 3T3-L1 cells.
Research Category
Signaling
Research Sub Category
PI3K, Akt, & mTOR Signaling

Quality

Routinely evaluated by immunoblot.

Target description

58 kDa

Physical form

100 μL of affinity purified rabbit polyclonal IgG in 50% storage buffer (PBS (without Mg2+ and Ca2+), pH 7.3 containing 1.0 mg/mL BSA (IgG, protease free) and 0.05% sodium azide) and 50% glycerol.
Affinity purified

Storage and Stability

Stable for 2 years at -20°C from date of shipment.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerolcontaining solutions to become frozen during storage.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

07-889:

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Ailen S Cervino et al.
Developmental neurobiology, 77(11), 1308-1320 (2017-07-19)
The olfactory epithelium (OE) has the remarkable capability to constantly replace olfactory receptor neurons (ORNs) due to the presence of neural stem cells (NSCs). For this reason, the OE provides an excellent model to study neurogenesis and neuronal differentiation. In
M E McMenamin et al.
Cancer research, 59(17), 4291-4296 (1999-09-15)
The tumor suppressor gene PTEN/MMAC-1/TEP-1 (referred to hereafter as PTEN) maps to chromosome 10q23 and encodes a dual specificity phosphatase. The PTEN protein negatively regulates cell migration and cell survival and induces a G1 cell cycle block via negative regulation
S Ramaswamy et al.
Proceedings of the National Academy of Sciences of the United States of America, 96(5), 2110-2115 (1999-03-03)
PTEN/MMAC1 is a tumor suppressor gene located on chromosome 10q23. Inherited PTEN/MMAC1 mutations are associated with a cancer predisposition syndrome known as Cowden's disease. Somatic mutation of PTEN has been found in a number of malignancies, including glioblastoma, melanoma, and
Thomas E Finger et al.
The Journal of comparative neurology, 525(7), 1743-1755 (2017-02-06)
The ionotropic serotonin receptor, 5-HT3 , is expressed by many developing neurons within the central nervous system. Since the olfactory epithelium continues to generate new olfactory sensory neurons (OSNs) throughout life, we investigated the possibility that 5-HT3 is expressed in
Olfactory deficits in Niemann-Pick type C1 (NPC1) disease.
Hovakimyan, M; Meyer, A; Lukas, J; Luo, J; Gudziol, V; Hummel, T; Rolfs, A; Wree, A; Witt, M
Testing null
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