07-577

Anti-Stargazin/Cacng2 Antibody, CT

Upstate^®, from rabbit

• Synonym(s): Neuronal voltage-gated calcium channel gamma-2 subunit, calcium channel, voltage-dependent, gamma subunit 2, voltage-dependent calcium channel gamma-2 subunit
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: rabbit
• Quality Level: 100
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: polyclonal
• species reactivity: rat, mouse
• manufacturer/tradename: Upstate^®
• technique(s): immunocytochemistry: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• isotype: IgG
• NCBI accession no.: NP_006069
• UniProt accession no.: Q9Y698
• shipped in: dry ice
• target post-translational modification: unmodified
• Gene Information: mouse ... Cacng2(12300) ; rat ... Cacng2(84347)

Description

General description

Stargazin belongs to a group called voltage gated calcium channels (VGCCs) which are present in most excitable cells. Stargazin is thought to stabilize the calcium channel in an inactivated (closed) state.
There are five high voltage activated calcium channel types (L, N, P, Q, and R) and one low voltage activated channel type (T). Each of these channels exists as a heteromultimer of a1, b, a2/ d and g subunits with the voltage activated calcium channel function carried by the a subunits. VGCCs exert spatial and temporal control over cellular calcium concentrations and serve to modulate neurotransmitter release, hormone secretion, muscle contraction, electrical activity, cell metabolism and proliferation, gene expression, and neuronal survival.

Specificity

Predicted to cross-react with mouse based on sequence similarity.

Recognizes Stargazin, MW ~36-39 kDa.

Immunogen

Epitope: C-terminus

KLH-conjugated, synthetic peptide corresponding to amino acids 305-323 (KDSKDSLHANTANRRTTPV) of rat Stargazin.

Application

Immunoprecipitation:
2-5 µg of this antibody has been reported by an independent laboratory to immunoprecipitate Stargazin in rat P2 lysate.

Research Category
Neuroscience

Research Sub Category
Synapse & Synaptic Biology

This Anti-Stargazin/Cacng2 Antibody, CT is validated for use in IC, IP, WB for the detection of Stargazin/Cacng2.

Quality

Evaluated by western blot on RIPA lysates from rat brain microsomal preparation (Catalog # 12-144).

Western Blot Analysis:
0.5-2.0 µg/mL of this antibody detected Stargazin in RIPA lysates from rat brain microsomal preparation (Catalog # 12-144).

Target description

~36-39 kDa

Physical form

Format: Purified

Protein A purified

Purified rabbit polyclonal IgG in buffer containing 0.1 M Tris-glycine, pH 7.4, 0.15 M NaCl, 0.05% sodium azide before the addition of glycerol to 30%.

Storage and Stability

Stable for 1 year at -20ºC from date of receipt.

Analysis Note

Control
Brain tissue

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 1