07-2139

Anti-Cysteine Sulfenic Acid Antibody

serum, from rabbit

• eCl@ss: 32160702
• UNSPSC Code: 12352203
• Clone: polyclonal
• Species reactivity: mouse, rat, human
• Application: WB
• Citations: 2

Properties

• biological source: rabbit
• antibody form: serum
• antibody product type: primary antibodies
• clone: polyclonal
• species reactivity: mouse, rat, human
• species reactivity (predicted by homology): mammals
• technique(s): western blot: suitable
• shipped in: wet ice
• target post-translational modification: unmodified

Description

General description

Multiple kDa are observed that increases with oxidized stress (H2O2).

Protein sulfenic acid formation is a reversible post-translational modification that may be used to monitor protein oxidation on reactive cysteines within target proteins. This can be detected with protein sulfenic acid derivatised with dimedone.

Immunogen

Epitope: Other

KLH-conjugated corresponding to cysteine sulfenic acid.

Application

Anti-Cysteine Sulfenic Acid detects levels of Cysteine Sulfenic Acid proteins & has been published & validated for use in WB.

Research Category
Apoptosis & Cancer

Research Sub Category
General Post-translation Modification

Western Blot Analysis: A previous lot of this antibody detected cysteine sulfenic acid in NIH/3T3 and HEK293 cell lysates.

Biochem/physiol Actions

This antibody recognizes proteins containing cysteine sulfenic acid derivitized with dimedone.

Physical form

Unpurified

Unpurified rabbit polyclonal with 0.05% sodium azide.

Preparation Note

Stable for 1 year at from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Analysis Note

Control
Rat ventricular myocyte lysate

Evaluated by Western Blot in rat ventricular myocyte lysate.

Western Blot Analysis: 1:10,000 dilution of this antibody detected cysteine sulfenic acid on 10 µg of rat ventricular myocyte lysate.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk: WGK 1