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Key Documents

Safety Information

07-1802

Sigma-Aldrich

Anti-TAF3 Antibody

serum, from rabbit

Synonym(s):

Transcription initiation factor TFIID subunit 3, 140 kDa TATA box-binding protein-associated factor, TBP-associated factor 3

Transcription initiation factor TFIID 140 kDa subunit, TAF(II)140, TAF140, TAF140, TAFII140

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

antibody form

serum

antibody product type

primary antibodies

clone

polyclonal

species reactivity

mouse

species reactivity (predicted by homology)

rhesus macaque, guinea pig, human

technique(s)

ChIP: suitable
immunoprecipitation (IP): suitable

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... TAF3(83860)
mouse ... Taf3(209361)

General description

TAF3 is a subunit of TFIID that was initially identified through its interaction with a developmentally regulated transcription factor Bric a brac 1 (bab1), which is implicated in pattern formation and tissue morphogenesis in Drosophila. TFIID is a general or basal transcription factor that regulates initiation by allowing RNA polymerase II to recognize a core promotor. TAF3-TBPL2 complexes are found in myoblasts; however, upon differentiation into myotubes, they are replaced by TRF3-TAF3 complexes. Further research is still required to elucidate the roles and the development of the former to the latter complex.

Immunogen

GST-tagged recombinant protein corresponding to human TAF3.

Application

Chromatin Immunoprecipitation: A representative lot immunoprecipitated TAF3 using chromatin from transfected cells grown under conditions that favor expression from the MyoD:EGFP transgene promoter over expression from the endogenous MyoD promoter.
Detect TAF3 using this Anti-TAF3 Antibody validated for use in Chromatin Immunoprecipitation (ChIP) and Immunoprecipitation.
Research Category
Epigenetics & Nuclear Function
Research Sub Category
Transcription Factors

Quality

Evaluated by Immunoprecipitation in C2C12 cell lysate.

Immunoprecipitation Analysis: A 1:1,000 dilution of this antibody detected TAF3 in 500 µg of C2C12 cell lysate.

Target description

~180 kDa observed.

Physical form

Rabbit polyclonal serum containing with 0.05% sodium azide.
Unpurified

Storage and Stability

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Analysis Note

Control
C2C12 cell lysate.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 1


Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

07-1802:


Certificates of Analysis (COA)

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