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07-1735

Sigma-Aldrich

Anti-HuR Antibody

from rabbit, purified by affinity chromatography

Synonym(s):

ELAV-like 1, Hu antigen R, Hu-antigen R, embryonic lethal, abnormal vision, drosophila, homolog-like 1

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

100

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

human, rat, mouse

technique(s)

immunofluorescence: suitable
western blot: suitable

NCBI accession no.

NP_001410.2

UniProt accession no.

Q15717

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... ELAVL1(1994)
mouse ... Elavl1(15568)
rat ... Elavl1(363854)

General description

HuR (Hu-antigen R, ELAV-like protein 1, ELAV1) is an RNA bindnig protein that contains 3 RNA-binding domains. It is known to binds AU-rich elements if mRNA. It regulates gene expression by destabilizing their mRNA. It is known to be upregulated in certain cancers such as breast and gastric cancers. Some of its targets include cFos, IL-3, and BRCA1 mRNAs. HuR has also been shown to be involved in the regulation of VEFG-A and NFkB expression in certain cell types. It activation and accumulation are also linked to many energy signaling molcules such as that of the PI3K/Akt pathway and AMPK.

Specificity

Detects HuR
Other species have not been tested.

Immunogen

Epitope: N-terminus
Synthetic peptide corresponding to the N-terminus of human HuR.

Application

Immunofluorescent ICC Analysis:
Research Category
Signaling
Research Sub Category
Insulin/Energy Signaling

RNA Binding Protein (RBP)
Use Anti-HuR Antibody (Rabbit Polyclonal Antibody) validated in WB, IF to detect HuR also known as ELAV-like 1, Hu antigen R.

Quality

Western Blot Analysis:
The antibody detected HuR at 1:500-1:1,000 dilutions in Jurkat cell lysate resolved via SDS-PAGE and transferred to PVDF.

Target description

36 kDa

Linkage

Replaces: AB5831

Physical form

Affinity purified polyclonal precipitated in ammonium sulfate.
Rabbit polyclonal precipitated antibody in a solution of 50% saturated ammonium sulfate and PBS containing no preservatives

Storage and Stability

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution.

Analysis Note

Control
Jurkat cell lysate

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

07-1735:

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Identification of Novel Stress Granule Components That Are Involved in Nuclear Transport.
Mahboubi, H; Seganathy, E; Kong, D; Stochaj, U
Testing null
Weirui Ma et al.
eLife, 10 (2021-03-03)
Liquid-like condensates have been thought to be sphere-like. Recently, various condensates with filamentous morphology have been observed in cells. One such condensate is the TIS granule network that shares a large surface area with the rough endoplasmic reticulum and is
Harsha Raheja et al.
PLoS pathogens, 19(8), e1011552-e1011552 (2023-08-04)
Host protein HuR translocation from nucleus to cytoplasm following infection is crucial for the life cycle of several RNA viruses including hepatitis C virus (HCV), a major causative agent of hepatocellular carcinoma. HuR assists the assembly of replication-complex on the
Binyamin D Berkovits et al.
Nature, 522(7556), 363-367 (2015-04-22)
About half of human genes use alternative cleavage and polyadenylation (ApA) to generate messenger RNA transcripts that differ in the length of their 3' untranslated regions (3' UTRs) while producing the same protein. Here we show in human cell lines
Weirui Ma et al.
Cell, 175(6), 1492-1506 (2018-11-20)
Approximately half of human genes generate mRNAs with alternative 3' untranslated regions (3'UTRs). Through 3'UTR-mediated protein-protein interactions, alternative 3'UTRs enable multi-functionality of proteins with identical amino acid sequence. While studying how information on protein features is transferred from 3'UTRs to

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