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Safety Information

07-146

Sigma-Aldrich

Anti-Histone H2A (acidic patch) Antibody

serum, Upstate®

Synonym(s):

H2A histone family, member Q, histone 2, H2ac, histone IIa, histone cluster 2, H2ac

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

100

antibody form

serum

antibody product type

primary antibodies

clone

polyclonal

species reactivity

yeast, chicken, human, rat, Saccharomyces cerevisiae, mouse

manufacturer/tradename

Upstate®

technique(s)

ChIP: suitable
western blot: suitable

isotype

IgG

NCBI accession no.

NP_003508

UniProt accession no.

Q16777

shipped in

dry ice

target post-translational modification

unmodified

Gene Information

human ... HIST2H2AC(8338)

General description

Histone H2A is one of the 5 main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N terminal tail H2A is involved with the structure of the nucleosomes of the ′beads on a string′ structure.

Specificity

Broad species cross-reactivity is expected.
Recognizes Histone H2A, Mr 14 kDa.

Immunogen

KLH-conjugated, synthetic peptide corresponding to amino acids 88-97 (IRNDEELNKL-C) of human Histone H2A.

Application

Anti-Histone H2A (acidic patch) Antibody is a Rabbit Polyclonal Antibody for detection of Histone H2A (acidic patch) also known as histone 2-H2ac, histone IIa & has been validated in ChIP & WB.
Immunofluorescence:
Recommend Anti-phospho-Histone H2A.X (Ser139), clone JBW301, catalog #05-636.
Research Category
Epigenetics & Nuclear Function
Research Sub Category
Histones

Quality

Routinely evaluated by western blot on acid extracts from HeLa cell lysate.

Western Blot Analysis:
1:1000-1:5000 dilutions of this lot detected Histone H2A (acidic patch) in acid extracts from HeLa cell lysate.

Target description

14 kDa

Physical form

Rabbit antiserum polyclonal IgG in buffer containing 0.05% sodium azide. Frozen solution.
Unpurified

Storage and Stability

Stable for 1 year at -20°C from date of receipt.
For maximum recovery of product, centrifuge the vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Analysis Note

Control
HeLa cell extract.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

07-146:

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Michael Papetti et al.
American journal of physiology. Gastrointestinal and liver physiology, 301(3), G508-G518 (2011-07-09)
Altered profiles of gene expression reflect the reprogramming of intestinal epithelial cells during their maturation along the crypt-luminal axis. To focus on genes important in this process, and how they in turn are regulated, we identified 14 transcripts commonly downregulated
Regulation of the activation of the Fanconi anemia pathway by the p21 cyclin-dependent kinase inhibitor.
Rego MA, Harney JA, Mauro M, Shen M, Howlett NG
Oncogene null
2-Amino-3-methylimidazo[4,5-f]quinoline (IQ) promotes mouse hepatocarcinogenesis by activating transforming growth factor-? and Wnt/?-catenin signaling pathways.
Xie, XL; Wei, M; Kakehashi, A; Yamano, S; Tajiri, M; Wanibuchi, H
Toxicological Sciences null
Active VSG expression sites in Trypanosoma brucei are depleted of nucleosomes.
Stanne TM, Rudenko G
Eukaryotic Cell null
The histone H2A variant macroH2A1 does not localize to the centrosome.
Friedman, N; Barzily-Rokni, M; Isaac, S; Eden, A
Testing null
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