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05-801

Sigma-Aldrich

Anti-trimethyl-Histone H3 (Lys36) Antibody, clone MC86, rabbit monoclonal

clone MC86, Upstate®, from rabbit

Synonym(s):

H3K36me3, Histone H3 (tri methyl K36)

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

100

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

MC86, monoclonal

species reactivity

chicken

manufacturer/tradename

Upstate®

technique(s)

ChIP: suitable
dot blot: suitable
inhibition assay: suitable (peptide)
western blot: suitable

isotype

IgG

NCBI accession no.

NP_003484

UniProt accession no.

Q16695

shipped in

wet ice

target post-translational modification

trimethylation (Lys36)

Specificity

Broad species cross-reactivity is expected.
Recognizes Histone H3 trimethylated on Lysine 36.

Immunogen

peptide containing the sequence GVme3KKP, in which me3K corresponds to trimethyl lysine 36 of human histone H3

Application

Research Category
Epigenetics & Nuclear Function
Research Sub Category
Histones
Use Anti-trimethyl-Histone H3 (Lys36) Antibody, clone MC86 (rabbit monoclonal antibody) validated in DB, WB, PIA to detect trimethyl-Histone H3 (Lys36) also known as H3K36me3, Histone H3 (tri methyl K36).

Quality

routinely evaluated by immunoblot on chicken Core Histones (Catalog #13-107); did not detect unmodified recombinant Histone H3 (Catalog #14-411)

Target description

~17 kDa

Physical form

Format: Purified
Protein A Purified immunoglobulin in a solution containing 0.07M Tris-glycine, 0.105M NaCl, pH 7.4, 0.035% sodium azide, 30% glycerol.
Protein A purified

Storage and Stability

Maintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Analysis Note

Control
Acid extracted proteins from HeLa cells

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

05-801:

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Jian Zhang et al.
The Plant journal : for cell and molecular biology, 64(4), 604-617 (2010-09-09)
MADS-box transcription factors are known for their roles in plant growth and development. The regulatory mechanisms of spatial and temporal specific expression of MADS-box genes and the function of MADS-box genes in other biological processes are still to be explored.
The Iws1:Spt6:CTD complex controls cotranscriptional mRNA biosynthesis and HYPB/Setd2-mediated histone H3K36 methylation.
Yoh, SM; Lucas, JS; Jones, KA
Genes & Development null
Mass spectrometry analysis of the variants of histone H3 and H4 of soybean and their post-translational modifications.
Wu, T; Yuan, T; Tsai, SN; Wang, C; Sun, SM; Lam, HM; Ngai, SM
BMC plant biology null
Guochun Zhu et al.
International journal of biological sciences, 18(14), 5522-5538 (2022-09-24)
Cathepsins play a role in regulation of cell function through their presence in the cell nucleus. However, the role of Cathepsin K (Ctsk) as an epigenetic regulator in osteoclasts remains unknown. Our data demonstrated that Ctsk-/-Mmp9-/- mice have a striking
Jun Wang et al.
Nature plants, 6(7), 823-837 (2020-06-24)
Histone demethylation is crucial for proper chromatin structure and to ensure normal development, and requires the large family of Jumonji C (JmjC)-containing demethylases; however, the molecular mechanisms that regulate the substrate specificity of these JmjC-containing demethylases remain largely unknown. Here
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