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05-655

Sigma-Aldrich

Anti-p21/WAF1/Cip1 Antibody, clone CP74

clone CP74, Upstate®, from mouse

Synonym(s):

Anti-CAP20, Anti-CIP1, Anti-MDA-6, Anti-P21, Anti-SDI1, Anti-WAF1, Anti-p21CIP1

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

100

antibody form

purified antibody

antibody product type

primary antibodies

clone

CP74, monoclonal

species reactivity

human

manufacturer/tradename

Upstate®

technique(s)

immunohistochemistry: suitable
western blot: suitable

isotype

IgG2bκ

NCBI accession no.

NM_000389

UniProt accession no.

P38936

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... CDKN1A(1026)

Specificity

Cip1

Immunogen

Recombinant full-length human Cip1 with an N-terminal histidine tag; epitope corresponds to amino acids 1-80 of full-length human Cip1

Application

Anti-p21/WAF1/Cip1 Antibody, clone CP74 is a high quality Mouse Monoclonal Antibody for the detection of p21/WAF1/Cip1 & has been validated in WB.
Research Category
Epigenetics & Nuclear Function
Research Sub Category
Cell Cycle, DNA Replication & Repair

Quality

routinely evaluated by immunoblot on nuclear extract from HeLa cells

Target description

21kDa

Physical form

0.1M Tris-glycine, pH 7.4, 0.15M NaCl, 0.05% sodium azide before the addition of glycerol to 30%
Format: Purified
Protein G Purified

Storage and Stability

2 years at -20°C

Analysis Note

Control
Positive Antigen Control: Catalog #12-309, Hela cell nuclear extract. Add an equal volume of Laemmli reducing sample buffer to 10 μL of extract and boil for 5 minutes to reduce the preparation. Load 20 μg of reduced extract per lane for minigels.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

05-655:

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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M Hall et al.
Oncogene, 11(8), 1581-1588 (1995-10-19)
A number of low molecular weight proteins have recently been identified that specifically inhibit the function of cyclin-dependent protein kinases in mammalian cells. These fall into two distinct families based on primary sequence comparisons and probable modes of action. Using
Heat shock protein 90 inhibitors attenuate LPS-induced endothelial hyperpermeability.
Chatterjee, A; Snead, C; Yetik-Anacak, G; Antonova, G; Zeng, J; Catravas, JD
American Journal of Physiology. Lung Cellular and Molecular Physiology null
Expression and function of EZH2 in synovial fibroblasts: epigenetic repression of the Wnt inhibitor SFRP1 in rheumatoid arthritis.
Trenkmann M, Brock M, Gay RE, Kolling C, Speich R, Michel BA, Gay S, Huber LC
Annals of the Rheumatic Diseases null
Tobias Åkerström et al.
Scientific reports, 6, 19546-19546 (2016-01-28)
Primary aldosteronism (PA) is the most common cause of secondary hypertension with a prevalence of 5-10% in unreferred hypertensive patients. Aldosterone producing adenomas (APAs) constitute a large proportion of PA cases and represent a surgically correctable form of the disease.
N-methyl-N'-nitro-N-nitrosoguanidine (MNNG) triggers MSH2 and Cdt2 protein-dependent degradation of the cell cycle and mismatch repair (MMR) inhibitor protein p21Waf1/Cip1.
Jascur, T; Fotedar, R; Greene, S; Hotchkiss, E; Boland, CR
The Journal of Biological Chemistry null
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