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05-1049

Sigma-Aldrich

Anti-Met (extracellular) Antibody, clone 4F8.2

clone 4F8.2, from mouse

Synonym(s):

HGF receptor, HGF/SF receptor, Met proto-oncogene tyrosine kinase, SF receptor, Scatter factor receptor, met proto-oncogene, met proto-oncogene (hepatocyte growth factor receptor), oncogene MET

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

100

antibody form

purified antibody

antibody product type

primary antibodies

clone

4F8.2, monoclonal

species reactivity

human

technique(s)

immunohistochemistry: suitable (paraffin)
western blot: suitable

isotype

IgG2bκ

NCBI accession no.

NP_001120972.1

UniProt accession no.

P08581

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... MET(4233)

Related Categories

General description

The proto-oncogene Met (c-Met, HGF/SF Receptor, HGFR) is a receptor tyrosine kinase that is activated upon Hepatocyte Growth Factor (HGF) binding. This stimulates the autophosphorylation on Tyr1234/Tyr1235 which leads to pleiotropic downstream signaling events in epithelial cells that stimulate multiple cellular processes including cell proliferation, growth, differentiation, cell migration/ invasion, and tumorigenesis. These effects are mediated by SH2 domain-containing proteins that bind to the C-terminal tail of c-Met. Chronic stimulation of Met on cancer cells is thought to play a role in metastasis and dysregulation of c-Met activity is directly associated with oncogenesis, making it an important cancer target.

Specificity

Detects Met on its extracellular domain.

Immunogen

Epitope: Extracelluar domain (N-term)
GST-fusion protein corresponding to amino acids 80-434 of human Met.

Application

Detect Met (extracellular) using this Anti-Met (extracellular) Antibody, clone 4F8.2 validated for use in IH(P) & WB.
Research Category
Signaling
Research Sub Category
Growth Factors & Receptors
Western Blot Analysis:
This lot detected Met at 1:1,000 dilution in HEK293 cell lysate resolved via SDS-PAGE and transferred to PVDF.

Immunohistochemistry Analysis :

Quality

Routinely evaluated by Western Blot.

Western Blot Analysis:
This lot detected Met at 1:1,000 dilution in HEK293 cell lysate resolved via SDS-PAGE and transferred to PVDF.

Target description

170 kDa

Linkage

Replaces: 05-237

Physical form

Format: Purified
Protein G Purified
Purified mouse monoclonal in 0.1M Tris-Glycine (pH7.4), 150mM NaCl with 0.05% NaN3.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

05-1049:

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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