03-188

p300 (catalytic domain) human recombinant

Human recombinant p300 (catalytic domain) expressed in E. Coli.

• Synonym(s): Human recombinant p300 (catalytic domain) expressed in E. Coli, Upstate^® human recombinant p300 (catalytic domain), p300 (catalytic domain) expressed in E. Coli
• UNSPSC Code: 12352200
• NACRES: NA.54

Properties

• biological source: Escherichia coli
• Quality Level: 100
• Assay: >75% (SDS-PAGE)
• mol wt: ~52 kDa
• manufacturer/tradename: Upstate^®
• technique(s): enzyme immunoassay: suitable

Description

General description

Human recombinant p300 (catalytic domain) expressed in E. Coli
Although originally termed histone acetyltransferases (HATs) for their lysine acetylation activity on histone N-terminal tails, p300 and its paralogue, CBP, have been shown to acetylate a variety of non-histone proteins including p53, DNA polymerase ß and nuclear import factors. p300/CBP acetylations play regulatory roles in transcription, DNA repair and replication, the cell cycle, p53 turnover, and nuclear import. In addition to functions which overlap with those of CBP, p300 has a number of unique functions, including a role in the induction of p21Waf1/Cip1 cell cycle inhibitor.

Specificity

>250 pmol/min/µg assayed as production of CoA-SH from AcCoA in the presence of a peptide comprising human p53 residues 368-386. CoA-SH is determined colorimetrically by reaction with DTNB (5,5′-Dithiobis (2-nitrobenzoic acid).

Application

Human recombinant p300 (catalytic domain) expressed in E. Coli.

Acetyltransferase assays
Enzyme Assays

Physical form

Format: Purified

In liquid containing 50 mM Tris/HCl, pH 8.0, 0.1 mM EDTA, 10% glycerol. Frozen solution.

Storage and Stability

Stable for 12 months at -80ºC from date of receipt. Avoid multiple freeze/thaw cycles.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 1