03-0207-00

SMC^® Human PD-1 High Sensitivity Immunoassay Kit

• Synonym(s): PD-1 Single Molecule Counting kit, PD-1 ultra-sensitive immunoassay, PD-1 immunoassay kit, Programmed cell death protein 1 kit, PD-1 kit
• UNSPSC Code: 12161503
• NACRES: NA.47

Properties

• species reactivity: human
• Quality Level: 200
• parameter: 50 μL sample volume (diluted 2-fold in standard diluent)
• assay range: intra-assay cv: ≤5%; (n=15); linearity: 80-117%; (Plasma); linearity: 91-135%; (Serum); recovery range: 53-100%; (Plasma); recovery range: 80-109%; (Serum); sensitivity: 1.74 pg/mL; standard curve range: 0.87-3,000 pg/mL; inter-assay cv: ≤14%; (n=15)
• technique(s): single molecule counting: suitable
• input: sample type plasma (K2 EDTA); sample type serum
• shipped in: wet ice
• storage temp.: 2-8°C

Description

General description

Programmed cell death protein 1 (PD-1), CD279, is a type I membrane protein found in T cells, B cells, and macrophages that binds Programmed cell death ligand 1 and 2 (PD-L1 and PD-L2). PD-1 is found in both soluble and insoluble forms. PD-1 regulates the body’s immune response through a variety of pathways, down-regulating immune system activity and suppressing T-cell inflammatory activity. PD-1 has been implicated as playing a role in cancers, autoimmune diseases, the immune response to viruses, and in immunological pathways related to brain repair. As an immune checkpoint, PD-1 promotes apoptosis of antigen-specific T-cells in lymph nodes and reduces apoptosis in regulatory T-cells. Blockers of the PD-1 pathway have been approved as cancer therapeutics.

Research Category: Cancer
Research Sub-Category: Immuno-Oncology

Specificity

This assay was tested for cross-reactivity with PD-L1. No cross-reactivity was detected with this analyte.

Application

Used for ultrasensitivite quantification of Human PD-1 in human serum and plasma.

Features and Benefits

This SMC^® kit features:

• Accurate and reproducible performance on the state-of-the-art SMCxPRO^® ultrasensitive immunoassay system.
• Compatiblity with automated liquid handling

Legal Information

SMC is a registered trademark of Singulex

SMCxPRO is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Pictograms: GHS08,GHS07
• Signal Word: Danger
• Hazard Statements: H302 + H312 + H332,H319,H360FD,H373,H412
• Precautionary Statements: P273 - P280 - P301 + P312 - P302 + P352 + P312 - P304 + P340 + P312 - P308 + P313
• Hazard Classifications: Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3 - Eye Irrit. 2 - Repr. 1B - STOT RE 2 Inhalation
• Target Organs: Respiratory Tract
• Storage Class Code: 6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects