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Safety Information

D2650

Sigma-Aldrich

Dimethyl sulfoxide

Dimethyl sulfoxide

Hybri-Max, sterile-filtered, BioReagent, suitable for hybridoma, ≥99.7%

Synonym(s):

DMSO

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100 ML
¥28,080

¥28,080

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100 ML
¥28,080

About This Item

Linear Formula:
(CH3)2SO
CAS Number:
Molecular Weight:
78.13
Beilstein:
506008
EC Number:
MDL number:
UNSPSC Code:
12191502
PubChem Substance ID:
NACRES:
NA.75
grade:
Hybri-Max
Assay:
≥99.7%
technique(s):
cell culture | hybridoma: suitable
cryopreservation: suitable
bp:
189 °C (lit.)
vapor pressure:
0.42 mmHg ( 20 °C)

¥28,080

List Price¥35,100Save 20%

In StockDetails


grade

Hybri-Max

Quality Level

vapor density

2.7 (vs air)

vapor pressure

0.42 mmHg ( 20 °C)

sterility

sterile-filtered

product line

BioReagent

Assay

≥99.7%

form

liquid

autoignition temp.

573 °F

expl. lim.

42 %, 63 °F

technique(s)

cell culture | hybridoma: suitable
cryopreservation: suitable

impurities

endotoxin, tested
≤0.1% water
water

refractive index

n20/D 1.479 (lit.)

bp

189 °C (lit.)

mp

16-19 °C (lit.)

solubility

H2O: miscible (completely)

density

1.10 g/mL (lit.)

SMILES string

CS(C)=O

InChI

1S/C2H6OS/c1-4(2)3/h1-2H3

InChI key

IAZDPXIOMUYVGZ-UHFFFAOYSA-N

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Application

DMSO is a polar aprotic solvent used in chemical reactions, in polymerase chain reactions (PCR) and as a cryoprotectant vitrification agent for the preservation of cells, tissues and organs. DMSO is used in cell freezing media to protect cells from ice crystal induced mechanical injury. It is used for frozen storage of primary, sub-cultured, and recombinant heteroploid and hybridoma cell lines; embryonic stem cells (ESC), and hematopoietic stem cells. DMSO is frequently used in the combinations with BSA or fetal bovine serum (FBS).

Packaging

100 mL in amber bottle
Glass ampoule packaging is now available under a new product number, D2653

Other Notes

This product is a Hybri-Max product. It is hybridoma tested and is assessed for suitability in cell freezing. This product is sterile filtered (0.2 micron) and tested for endotoxin levels.

Caution

Supercools easily and remelts slowly at room temperature. Solidified product can be re-liquified by warming to room temperature without detriment to the product.

Legal Information

Hybri-Max is a trademark of Sigma-Aldrich Co. LLC

related product

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

188.6 °F - closed cup

Flash Point(C)

87 °C - closed cup

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

FSL

Group 4: Flammable liquids
Type 3 petroleums
Hazardous rank III
Water soluble liquid

ISHL Indicated Name

Substances Subject to be Indicated Names

ISHL Notified Names

Substances Subject to be Notified Names

JAN Code

D2650-1L:4548173193991
D2650-5X10ML:4548173194011
D2650-5X5ML:4548173194028
D2650-100ML:4548173193984
D2650-500ML:4548173194004
D2650-VAL:
D2650-PROC:
D2650-100ML-C:
D2650-VAR:
D2650-BULK:
D2650-5X5ML-C:


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Certificates of Analysis (COA)

Lot/Batch Number

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Azadeh Ghaee et al.
Materials science & engineering. C, Materials for biological applications, 103, 109767-109767 (2019-07-28)
In this study, a simple one-step method was introduced to prepare suitable PCL wound dressing by using a small amount of hydrophilic surface modifying macromolecule (LSMM) with ends capped with Polyethylene glycol and aminated zeolitic imidazolate frameworks (MZIF-8) as a
Stepan Coufal et al.
Cells, 8(7) (2019-07-25)
Crohn's disease (CD), ulcerative colitis (UC) and inflammatory bowel disease (IBD) associated with primary sclerosing cholangitis (PSC-IBD), share three major pathogenetic mechanisms of inflammatory bowel disease (IBD)-gut dysbiosis, gut barrier failure and immune system dysregulation. While clinical differences among them
Shady Sayed et al.
Methods (San Diego, Calif.), 164-165, 36-48 (2019-05-13)
The CRISPR/Cas9 system is transforming many biomedical disciplines, including cancer research. Through its flexible programmability and efficiency to induce DNA double strand breaks it has become straightforward to introduce cancer mutations into cells in vitro and/or in vivo. However, not
Hongxiu Diao et al.
BMC veterinary research, 15(1), 276-276 (2019-08-04)
Mammary gland tumor is the most common spontaneous tumor in intact female dogs, and its poor prognosis remains a clinical challenge. Ivermectin, a well-known anti-parasitic agent, has been implicated as a potential anticancer agent in various types of human cancer.
Likun Gao et al.
Cell death & disease, 10(8), 609-609 (2019-08-14)
Paeonol (Pae), a phenolic acid compound isolated from the Moutan Cortex, was previously demonstrated to exert multiple anticancer effects. The rational control of autophagy has been considered a potential treatment strategy for epithelial ovarian cancer. However, whether Pae induces autophagy

Articles

Overview of good cell banking practices for cell line cryopreservation purposes.

Development of a novel serum-free and xeno-free human mesenchymal stem cell (MSC) osteocyte differentiation media.

Protocols

Human ips cell passaging protocol using EZ-LiFT™ Reagent, an enzyme-free and chemically defined pluripotent stem cell dissociation reagent.

Cell culture protocol for high cell viability freezing using cryopreservation reagents like DMSO. Free handbook download.

Step-by-step stem cell culture protocols for human induced pluripotent stem cells (iPSCs) including ips cell thawing, expanding, freezing and characterizing.

Stem cell reprogramming protocols to generate human induced pluripotent stem cells (iPSCs) including viral and non-viral RNA based methods.

See All

Questions

1–10 of 17 Questions  
  1. Is it safe in terms of health and product safety to store a leftover DMSO ampule covered with parafilm in a flammable cabinet?

    1 answer
    1. DMSO is hygroscopic and light-sensitive. The storage container must protect from light and moisture uptake. Since this DMSO is sterile-filtered and intended for cell culture applications, the storage containers must also be sterile and endotoxin-free. It is recommended to purge aliquots and store them under inert gas, in a compatible, tightly sealed container, at a cool storage area, such as 4°C.

      DMSO is not compatible with many types of plastics, so it is advisable to store it in appropriate amber glass vials for long-term storage. Please refer to the Product Information Sheet and the Safety Data Sheet at the link below for compatibility of DMSO with various materials and for further details:

      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/944/265

      Helpful?

  2. Is this anhydrous?

    1 answer
    1. This product is not considered anhydrous. The maximum allowable water content is 0.1% which is reported on the lot-specific Certificate of Analysis. Please see the link below to review the anhydrous DMSO products available:
      https://www.sigmaaldrich.com/search/dmso-anhydrous?focus=products&page=1&perpage=30&sort=relevance&term=DMSO%20anhydrous&type=product

      Helpful?

  3. I ordered 10 ml glass vials, used 7 ml, Can I store it in centrifuge tubes? For how long can I use that?

    1 answer
    1. It may be best to store DMSO in amber glass vials for long term storage, as this compound is not compatible with many types of plastics. The compatibility of DMSO with various materials is listed below:
      Compatible: LDPE, HDPE, polypropylene, PPCO(polypropylene copolymer), polymethylpentene, nylon, teflon FEP
      Moderately compatible: polystyrene, ECTFE/ETFE
      Incompatible: polysulfone, flexible and rigid PVC tubing, polycarbonate

      Note that DMSO is hygroscopic and light sensitive. The storage container selected must offer protection from light and moisture uptake. The product is assigned a firm expiration date. Please refer to the lot specific Certificate of Analysis to assume the material is within the expected shelf life.

      Helpful?

  4. E' solo ad uso per ricerca o utilizzabile anche in diagnostica/human? Grazie

    1 answer
    1. This product is not suitable for diagnostic/human use.

      Helpful?

  5. Is this product packaged in a bottle with a septa cap?

    1 answer
    1. No, this product is not packaged with a septa cap. It comes in a 125 ml amber glass bottle with a 28 mm solid white cap.

      Helpful?

  6. Can I use it for PCR?

    1 answer
    1. While this product is tested specifically for use in cell culture, there is no reason to suspect that it would not perform well in PCR applications. The product is NOT tested for amplification, DNAse, or nuclease activity. Should this testing be important, see the link below to product options that include this testing:
      D9170 DMSO PCR Reagent:
      https://www.sigmaaldrich.com/product/sigma/d9170
      D8418 DMSO Molecular Biology Tested
      https://www.sigmaaldrich.com/US/en/product/sigma/d8418

      Helpful?

  7. what is the recommended re-test date & for how long can it be used beyond the expiry date mentioned in the COA?

    1 answer
    1. This product is assigned a firm expiration date of approximately 2 years. It is not assigned a retest date. The stability beyond the lot-specific expiration date is not determined and the quality of the product cannot be guaranteed. See the link below to review a sample Certificate of Analysis: https://www.sigmaaldrich.com/coa/SIGMA/D2650/RNBK5742.

      Helpful?

  8. Does DMSO have any synthetic utility?

    1 answer
    1. DMSO has been used to oxidize thiols and disulfides to sulfonic acids, hydrolysis of epoxides, thioalkylation of phenols, and oxidation of primary alcohols and halides, and esters of primary alcohols to aldehydes.

      Helpful?

  9. What is the molarity of DMSO?

    1 answer
    1. DMSO will have a molarity of 14.1M, based on a density of 1.1 g/mL and a molecular weight of 78.13 g/mol.

      Helpful?

  10. What type of filter is used to sterile-filter Dimethyl sulfoxide (DMSO)?

    1 answer
    1. DMSO can be sterile-filtered through a 0.2 micron PTFE filter. The sterility testing is per the current US Pharmacopoeia method.

      Helpful?

1–10 of 17 Questions  

Reviews

Active Filters

  1. Turkey
    • Review 1
    • Votes 3
    5 out of 5 stars.

    Is this product already concentration adjusted

    Is this product already concentration adjusted, as you mentioned this product used %8.7 concentration bu the thing i cannot understand that is this item already diluted for ready to use or do we have to dilute it what concentration we need ?

    Helpful?

  2. 9 Ratings-Only Reviews

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