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T7799

Sigma-Aldrich

Tumor Necrosis Factor-β human

>95% (SDS-PAGE), recombinant, expressed in E. coli, powder, suitable for cell culture | mammalian: suitable

Synonym(s):

TNF-β

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About This Item

MDL number:
UNSPSC Code:
12352202
NACRES:
NA.77

product name

Tumor Necrosis Factor-β human, TNF-β, recombinant, expressed in E. coli, powder, suitable for cell culture

biological source

human

Quality Level

recombinant

expressed in E. coli

Assay

>95% (SDS-PAGE)

form

powder

potency

4-20 pg/mL Activity

quality

endotoxin tested

mol wt

protein 18.8 kDa

packaging

pkg of 10 μg

storage condition

avoid repeated freeze/thaw cycles

technique(s)

cell culture | mammalian: suitable

impurities

≤1 EU/μg

UniProt accession no.

storage temp.

−20°C

Gene Information

Biochem/physiol Actions

Tumor necrosis factor-β (TNF-β), also known as lymphotoxin-α (LT-α) and cytotoxic factor, is a 25 kDa glycoprotein that is tightly regulated by lymphocytes. TNF-β and TNF-α share a 28% amino acid sequence identity, and as soluble forms, both can exist in solution as homotrimers, activate the same TNF receptors (TNFR1 and TNFR2) and elicit many of the same bioactivities. Unlike TNF-α, TNF-β can also form heterotrimers with one or two molecules of the related membrane-bound lymphotoxin-β (LT-β). Reportedly, LTα1/β2, the predominant heterotrimer, binds and activates only the LT-β receptor, LT-βR. The other heterotrimer, LT-α2/β1 binds and activates TNFR1 and TNFR2 but not LT-βR. TNF-β is expressed in activated T, B and NK cells and in certain transformed cells. Genes for TNF-β, LT-β and TNF-α are tightly linked near each other within the MHC. TNF-β appears to play many of the same biological roles as TNF-α, but the membrane-bound form with LT-β is critical for normal lymphoid organ development. Genetic polymorphisms in TNF-α and TNF-β have been linked to certain pathological conditions, including myasthenia gravis. At the amino acid level, human and mouse TNF-β are 74% homologous and human and mouse LT-β are 80% homologous.

Physical form

lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein

Analysis Note

The biological activity is measured in a cytotoxicity assay using the TNF-suspectible murine L929 cell line in the presence of actinomycin D.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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O Jónasdóttir et al.
APMIS : acta pathologica, microbiologica, et immunologica Scandinavica, 109(11), 781-786 (2002-03-20)
Wegener's granulomatosis (WG) is a systemic inflammatory disease with vasculitis as the key feature. Abnormal expression of tumour necrosis factor alpha (TNFalpha) is considered of prime pathogenic importance in several inflammatory diseases. The effects of TNFa are mediated by TNF
H Gutman et al.
International journal of molecular medicine, 8(2), 199-203 (2001-07-11)
Tumor necrosis factor (TNF) causes cell necrosis in vivo by damaging the endothelium of the neovasculature. However, its mechanism of action is not well understood. We hypothesized that TNF affects the tumor microenvironment even before neovascularization occurs, thereby increasing lymphocyte

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