P1473

PCR 100 bp Low Ladder

for electrophoresis of PCR fragments

• Synonym(s): 100bp maker, 100bp marker for agarose gel electrophoresis, 100pb ladder for agarose gel electrophoresis, DNA ladder, DNA marker, gel ladder, gel marker
• NACRES: NA.25
• UNSPSC Code: 41141827

Properties

• Product Name: PCR 100 bp Low Ladder, for electrophoresis of PCR fragments
• biological source: synthetic
• grade: Molecular Biology
• form: liquid
• usage: 75 uses
• suitability: suitable for electrophoresis (DNA)
• storage temp.: −20°C
• Quality Level: 200

Description

Application

PCR 100 bp Low Ladder has been used:

• in agarose gel electrophoresis as a standard DNA marker to obtain DNA fragments of about 240 bp for polymerase chain reaction (PCR)-denaturing gradient gel electrophoresis (DGGE) analysis[1]
• to visualize DNA from enzyme digested PCR mixture[2]
• to estimate the molecular weight of the PCR product fragments[3]

General description

Sigma′s PCR 100 bp Low Marker contains 10 dsDNA fragments from 100 – 1,000 bases, evenly spaced in 100 bp repeats. Two ul of the ladder should be diluted in gel loading buffer and then loaded in a single lane on an agarose gel.

The PCR 100 bp low ladder has been specially designed for the size determination of polymerase chain reaction (PCR) generated DNA fragments.

Other Notes

Contains 10 bands, 100–1,000 bp in exact 100 bp spaced (ladder) recombinant repeats.

For optimal resolution, the recommended agarose gel concentration is 2.0%.

Sigma′s PCR 100 bp Low Marker is provided in a solution of 10 mM Tris-HCl (pH 8.0), with 1.0 mM EDTA.

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)