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MBD0063

Sigma-Aldrich

Vitamin Mixture Solution

Ready-made microbiology growth media supplement

Synonym(s):

Vitamin mix

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About This Item

UNSPSC Code:
41106212
NACRES:
NA.75

Quality Level

sterility

0.2 μm filtered

form

liquid

packaging

pkg of 10 mL

concentration

100 ×

suitability

suitable for (Media supplement)

shipped in

dry ice

storage temp.

-10 to -25°C

General description

Vitamin mixtures are used regularly in promoting growth of bacteria and fungi from different types of microbiomes, such as gut, rumen, soil, water, etc,. The use of vitamin mixtures helps scientists reduce time spent on creating the optimal conditions for microbial growth of a specific bacteria as well as diversified culturing of microbial samples. Different types of bacteria (aerobic, anaerobic, extremophiles, etc.) require different combinations of a variety of vitamins to improve their culturability. These include water-soluble vitamins, such as the B vitamins which are a broad category of small molecules that are important for cell metabolism but otherwise do not necessarily share structural or functional characteristics. In microbial studies the use of sequencing techniques is very common for identification of microorganisms in a certain habitat or niche. However, there is a discrepancy between the diversity that one can identify by culture techniques and by sequencing techniques. While the diversity of bacterial species identified by NGS techniques is very high, the number of culturable bacteria and fungi is much lower. With the growth of microbial research, new efforts to optimize culture-based techniques now allow the cultivation of previously uncultivated microbiome bacteria,.

Application

Vitamins, additives, and mineral supplements are used in special culturomics growth media that mimic the natural environment of bacteria and fungi for high throughput culturing. Vitamin mixture solutions contain nutrients and cofactors necessary to support growth from different types of microbial environments like human/animal microbiome, environmental microbiome, as well as other industries such as health and nutrition that rely on microbial research and diagnostics.

Features and Benefits

  • Convenient readymade solution to speed up media preparation
  • Sterile solution
  • Optimal mixture for growth of fastidious bacteria as well as diversified culturing of microbial samples

Components

  • Biotin : 2 mg/L
  • Folic acid:2 mg/L
  • Pyridoxamine-HCl: 10 mg/L
  • Thiamine-HCl x 2 H2O: 5mg/L
  • Riboflavin: 5mg/L
  • Nicotinic acid: 5mg/L
  • D-Ca-pantothenate: 5mg/L
  • Cyanocobalamine:0.1mg/L
  • p-Aminobenzoic acid: 5mg/L
  • Lipoic acid:5mg/L
  • KH2PO4:900mg/L

Storage Class Code

12 - Non Combustible Liquids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Culturing the human microbiota and culturomics
Nature Reviews. Microbiology, 16, 540-550 (2018)
Vitamins for the Gut Microbiome
Trends in Molecular Medicine, 26(2), 137-140 (2020)
Environmental electroactive consortia as reusable biosensing element for freshwater toxicity monitoring
New Biotechnology, 55, 36-45 (2020)
Serpentinimonas gen. nov., Serpentinimonas raichei sp. nov., Serpentinimonas barnesii sp. nov. and Serpentinimonas maccroryi sp. nov., hyperalkaliphilic and facultative autotrophic bacteria isolated from terrestrial serpentinizing springs
International Journal of Systematic and Evolutionary Microbiology, 71(8), 004945-004945 (2021)
A Defined Medium for Cultivation and Exometabolite Profiling of Soil Bacteria
Frontiers in Microbiology, 13, 855331-855331 (2022)

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