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I2002

Isocitric Dehydrogenase (NADP) from porcine heart

Name: Isocitric Dehydrogenase (NADP) from porcine heart
Brand: SIGMA

Type IV, buffered aqueous glycerol solution, 3-20 units/mg protein

Synonym(s):

threo-D_s-Isocitrate: NADP⁺ oxidoreductase, ICDH, IDH

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About This Item

CAS Number:

9028-48-2

UNSPSC Code:

12352204

NACRES:

NA.54

EC Number:

200-289-5

MDL number:

MFCD00131420

EC Number:

1.1.1.42 (BRENDA, IUBMB)

Specific activity:

3-20 units/mg protein

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Properties

type

Type IV

form

buffered aqueous glycerol solution

quality

≤ 0.2% of main activity is obtained with NAD as cofactor.

specific activity

3-20 units/mg protein

composition

Protein biuret

shipped in

wet ice

storage temp.

2-8°C

Description

Application

Isocitric dehydrogenase (IDH) has been used:

for in vitro activity assay to study the effects of thorium on citric acid cycle enzymes in the mitochondrial respiration pathway
as a standard to measure the impact of temperature on nicotinamide diphosphate (NADP)-IDH activity
in isocitrate assay

Biochem/physiol Actions

Isocitrate dehydrogenase (IDH) is a homodimeric enzyme and participates in several processes such as adapting to hypoxia, histone demethylation, and DNA modification. This enzyme has three isoforms like IDH₁, IDH₂, and IDH₃.

Physical form

Solution in 50% glycerol in EDTA buffer salts, pH 6.0

Other Notes

One unit will convert 1.0 μmole of isocitrate to α-ketoglutarate per min at pH 7.4 at 37 °C.

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Storage Class

10 - Combustible liquids

wgk

WGK 2

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

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Iron-sulfur cluster deficiency can be sensed by IRP2 and regulates iron homeostasis and sensitivity to ferroptosis independent of IRP1 and FBXL5.

Erdem M Terzi et al.

Science advances, 7(22) (2021-05-28)

Intracellular iron levels are strictly regulated to support homeostasis and avoid iron-mediated ROS production. Loss of iron-sulfur cluster (ISC) synthesis can increase iron loading and promote cell death by ferroptosis. Iron-responsive element-binding proteins IRP1 and IRP2 posttranscriptionally regulate iron homeostasis.

Interactions between transhydrogenase and thio-nicotinamide Analogues of NAD(H) and NADP(H) underline the importance of nucleotide conformational changes in coupling to proton translocation.

Avtar Singh et al.

The Journal of biological chemistry, 278(35), 33208-33216 (2003-06-07)

Transhydrogenase couples the reduction of NADP+ by NADH to inward proton translocation across mitochondrial and bacterial membranes. The coupling reactions occur within the protein by long distance conformational changes. In intact transhydrogenase and in complexes formed from the isolated, nucleotide-binding

Icksoo Lee et al.

Biochimica et biophysica acta, 1802(2), 275-283 (2009-10-20)

Noonan syndrome (NS) is an autosomal dominant disorder, and a main feature is congenital heart malformation. About 50% of cases are caused by gain-of-function mutations in the tyrosine phosphatase SHP2/PTPN11, a downstream regulator of ERK/MAPK. Recently it was reported that

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Global Trade Item Number

SKU	GTIN
I2002-25MG	04061832730448
I2002-250MG	04061832730431
I2002-25G-A	04061833848241
I2002-5G-A	04061832490205
I2002-100MG	04061833848234
I2002-500MG	04061832730455