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I0406

Sigma-Aldrich

Interleukin-6 from rat

IL-6, recombinant, expressed in E. coli, buffered aqueous solution, suitable for cell culture

Synonym(s):

Interleukin-6 from rat

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About This Item

MDL number:
MFCD01323739
UNSPSC Code:
12352202
NACRES:
NA.77

biological source

rat

Quality Level

200

recombinant

expressed in E. coli

Assay

>97% (SDS-PAGE)

form

buffered aqueous solution

potency

0.01-0.06 ng/mL ED50

quality

endotoxin tested

mol wt

predicted mol wt ~22 kDa

packaging

pkg of 10 and 50 μg

technique(s)

cell culture | mammalian: suitable

impurities

<0.01EU/mg (LAL test)

UniProt accession no.

P20607.1

shipped in

dry ice

storage temp.

−70°C

Gene Information

rat ... Il6(24498)

Biochem/physiol Actions

Interleukin-6 (IL-6) is a multifunctional protein originally discovered in the media of cells stimulated with double stranded RNA. IL-6 appears to be directly involved in the responses that occur after infection and injury and may prove to be as important as IL-1 and TNF-α in regulating the acute phase response. IL-6 is reported to be produced by fibroblasts, activated T cells, activated monocytes or macrophages, and endothelial cells. It acts upon a variety of cells, including fibroblasts, myeloid progenitor cells, T cells, B cells and hepatocytes. IL-6 induces multiple effects, as indicated by its numerous synonyms: plasmacytoma growth factor (PCT-GF), interferon-β-2 (IFN-β2), monocyte derived human B cell growth factor, B cell stimulating factor (BSF-2), hepatocyte stimulating factor (HSF), Interleukin Hybridoma/Plasmacytoma-1 (IL-HP1). In addition, IL-6 appears to interact with IL-2 in the proliferation of T lymphocytes. IL-6 also potentiates the proliferative effect of IL-3 on multipotential hematopoietic progenitors.

Physical form

Supplied as a 0.2 μm filtered solution in 50 mM sodium acetate, pH 4, with BSA as a carrier protein.

Preparation Note

Recombinant, rat Interleukin-6 is produced from a DNA sequence encoding the mature rat IL-6 protein. The methionyl form of mature rat IL-6, a 188 amino acid protein, has a predicted molecular mass of approx. 22 kDa. Rat and mouse IL-6 share 93% amino acid sequence identity.

Analysis Note

The biological activity is measured in a cell proliferation assay using IL-6 dependent mouse plasmacytoma cell line, T1165.85.2.1.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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P W Bodell et al.
Journal of applied physiology (Bethesda, Md. : 1985), 106(2), 443-453 (2008-12-06)
Childhood diseases are often accompanied by chronic inflammation, which is thought to negatively impact growth. Interleukin-6 (IL-6) is typically cited as an indicator of inflammation and is linked to impaired growth. This study was designed to isolate and identify potential
Elin Banke et al.
Metabolism: clinical and experimental, 63(6), 831-840 (2014-04-02)
The bacteria Staphylococcus aureus is part of the normal bacterial flora and produces a repertoire of enterotoxins which can cause food poisoning and toxic shock and might contribute to the pathogenesis of inflammatory diseases. These enterotoxins directly cross-link the T
Lorena M Amaral et al.
American journal of obstetrics and gynecology, 211(2), 158-158 (2014-02-20)
Preeclampsia is a multisystem disorder recognized as hypertension with proteinuria developing >20 weeks' gestation. Preeclampsia is associated with chronic immune activation characterized by increased T and B lymphocytes, cytokines, and antibodies activating the angiotensin II type I receptor (AT1-AA). Hypertension
Mingming Zhang et al.
PloS one, 9(10), e109146-e109146 (2014-10-03)
It has been shown that Faecalibacterium prausnitzii (F. prausnitzii), one of the dominant intestinal bacterial flora, may protect colonic mucosa against the development of inflammation and subsequent inflammatory bowel disease (IBD), with the underlying mechanisms being unclear. The impacts of
A O'Toole et al.
British journal of cancer, 111(5), 927-932 (2014-07-25)
Tumour microenvironment (TME) of advanced colorectal cancer (CRC) suppresses dendritic cell (DC) maturation. Here, our aim was to determine how the microenvironment of early-stage tumours influences DCs. Tumour-conditioned media (TCM) was generated by culturing explant tumour tissue in vitro (n=50).
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