H7041

IL-2 human

≥98% (SDS-PAGE), recombinant, expressed in HEK 293 cells, lyophilized powder, suitable for cell culture

• Synonym(s): Interleukin-2, T-cell growth factor
• MDL number: MFCD00165721
• UNSPSC Code: 12352202
• NACRES: NA.77

Properties

• Product Name: Interleukin-2 human, IL-2, recombinant, expressed in HEK 293 cells, suitable for cell culture, endotoxin tested
• biological source: human
• Quality Level: 100
• recombinant: expressed in HEK 293 cells
• form: lyophilized powder
• potency: ≤5.0 ng/mL ED₅₀
• quality: endotoxin tested
• mol wt: monomer 15 kDa (glycosylated)
• packaging: pkg of 5X10 μg; pkg of 10 μg; pkg of 100 μg
• technique(s): cell culture | mammalian: suitable
• UniProt accession no.: P60568
• storage temp.: −20°C
• Gene Information: human ... IL2(3558)

Description

General description

Interleukin-2 (IL-2) is an α-helical cytokine belonging to T-helper cell type 1. It has a molecular weight of 15kDa.[1] The IL2 gene is mapped to human chromosome 4q27.[1] IL-2 is originally called as T cell growth factor.[2]

Application

Interleukin-2 human has been used to culture peripheral blood mononuclear cells (PBMC).[3] It has been used to activate effector cell (PBMC) and to study antibody-dependent cell-mediated cytotoxicity.[4]

Biochem/physiol Actions

Interleukin-2 (IL-2) is an immunomodulatory factor produced by certain subsets of T lymphocytes. This lympho­kine promotes long term growth of acti­vated T cells and related cell types. IL-2 plays a role in the activation and proliferation of NK cells, induces γ-inter­feron and B cell growth factor secretion, and modulates the expression of the IL-2 receptor.

Interleukin-2 (IL-2) is essential for proliferation of natural killer (NK) cells, lymphocytes and macrophages.[1] It plays a key role in the immune system functions such as NK cell-stimulated immune responses. IL-2 also mediates the formation[5] and essential functions of regulatory T cells. It is implicated in autoimmune disease.[6] IL-2 is associated with apoptosis and thus, possesses antitumor response.[5] IL-2 mediates CD41⁺ T-cells differentiation into T-helper cell 1 and 2 effector subsets, preventing T-helper 17 differentiation.[1]

Preparation Note

Human Interleukin-2 (IL-2) expressed in human HEK 293 cells is a glycosylated monomer with a molecular mass of 15 kDa. Production in human HEK 293 cells offers authentic glycosylation. Glycosylation contributes to stability in cell growth media and other applications.

Analysis Note

The specific activity was determined by the dose-dependent stimulation of the proliferation of mouse CTLL-2 cells (mouse cytotoxic T cell line).

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable