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F6008

Sigma-Aldrich

Ficin from fig tree latex

lyophilized powder

Synonym(s):

Debricin, Ficain, higueroxyl delabarre

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About This Item

CAS Number:
Enzyme Commission number:
EC Number:
MDL number:
UNSPSC Code:
12352204
NACRES:
NA.54

biological source

fig tree (latex)

Quality Level

form

lyophilized powder

specific activity

≥1.0 units/mg protein

mol wt

23.8 kDa

composition

Protein, 80-100% biuret

storage temp.

−20°C

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General description

Extinction Coefficient: E1% = 21.0 (280 nm)
pI: 9.0

Application

Ficin from fig tree latex has been used:
  • in the proteolysis of tendon collagen
  • in the digestion of Chlorophyta Ulva rigida
  • to investigate it effect on protease-activated receptors 2 and 4 activation to induce itch in skin

Biochem/physiol Actions

Ficin is classified as a thiol protease. It contains a single reactive cysteine at its active site. The amino acid homology of the active site is similar to that of papain. Ficin will cleave proteins at the carboxyl side of Gly, Ser, Thr, Met, Lys, Arg, Tyr, Ala, Asn, and Val. The reported Km for the chromogenic substrate pGlu-Phe-Leu-p-nitroanilide is 0.43 mM. Ficin is inhibited by iodoacetamide, iodoacetic acid, N-ethylmaleimide, mercuric chloride, DFP (diisopropyl fluorophosphate), TLCK (Na-p-Tosyl-lysine chloromethyl ketone), and TPCK (N-Tosyl-L-phenylalanine chloromethyl ketone). Ficin can be used to generate high yielding F (ab′)2 fragments from mouse IgG1.
Ficin is effective in disrupting the staphylococcal biofilms making it accessible antibiotics exposure.

Unit Definition

One unit will produce a ΔA280 of 1.0 per min at pH 7.0 at 37°C when measuring TCA soluble products from casein in a final volume of 10 ml (1 cm light path).

Physical form

Lyophilized powder containing L-cysteine and trace EDTA

Preparation Note

This enzyme is soluble in water (10 mg/ml), yielding a clear solution.

inhibitor

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Description
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substrate

Product No.
Description
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Pictograms

Health hazardExclamation mark

Signal Word

Danger

Hazard Statements

Hazard Classifications

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

Target Organs

Respiratory system

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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A Aktypis et al.
Journal of applied microbiology, 84(4), 568-576 (1998-06-20)
ACA-DC 0040 produced an antimicrobial agent, which was named thermophilin T, active against several lactic acid bacteria strains of different species and food spoilage bacteria, such as Clostridium sporogenes C22/10 and Cl. tyrobutyricum NCDO-1754. The crude antimicrobial compound is sensitive
Targeting microbial biofilms using Ficin, a nonspecific plant protease
Baidamshina DR, et al.
Scientific reports, 7, 46068-46068 (2017)
Generation of an artificial skin construct containing a non-degradable fiber mesh: a potential transcutaneous interface
Cahn F and Kyriakides TR
Biomedical Materials (Bristol, England), 3(3), 034110-034110 (2008)
Yusei Takemoto et al.
Journal of cellular physiology, 234(2), 1689-1698 (2018-08-03)
Nuclear factor erythroid 2-related factor 2 (Nrf2) is a master regulator for the induction of antioxidative genes and plays roles in diverse cellular functions. The roles of Nrf2 in muscle regeneration have been investigated, and both important and unimportant roles
Isolation and characterization of angiotensin I-converting enzyme (ACE) inhibitory peptides from Ulva rigida C. Agardh protein hydrolysate
Paiva L, et al.
Journal of functional foods, 26, 65-76 (2016)

Protocols

This procedure may be used for all Ficin products.

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